TY - JOUR AB - Colon cancer is the main cause of cancer mortality worldwide. Its poor prognosis is mainly ascribed to high recurrence rates. Identifying novel prognostic biomarkers and therapeutic key points for management is crucial and important. Long non-coding RNAs (lncRNAs) are a class of RNAs, which have various roles in carcinogenicity and molecular mechanisms. The lncRNA small nucleolar RNA host gene 1 (SNHG1) contributes to the promotion of tumor development, however, the connections between SNHG1 and colon cancer are still unclear. The aim of the present study was to investigate the clinical significance, the biological functions, and the potential mechanism of SNHG1 in colon cancer. In the present study, we referred to the Oncomine database and used RT-qPCR to determine that SNHG1 expression was significantly higher both in colon cancer tissues and cancerous cell lines than in normal samples. Cell functional experiments were performed after knockdown of SNHG1, including Cell Counting Kit-8 assay, colony formation assay, Transwell® assay, and flow cytometric analyses of cell apoptosis, which suggested that SNHG1 stimulated colon cancer cell proliferation, promoted cell invasion and migration, and inhibited apoptosis. Immunohistochemical staining and western blotting experiments revealed that in colon cancer cells with SNHG1 knockdown, β-catenin, c-Myc and cyclin D1 protein levels were decreased, while E-cadherin was increased, which suggested that SNHG1 promoted colon cancer cell proliferation, migration and invasion through the Wnt/β-catenin signaling pathway. Our results indicated that SNHG1 and its interrelated components may be future therapeutic targets of carcinoma of the colon. AD - Department of Gastroenterology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China Department of Gastroenterology, Chongqing People's Hospital, Chongqing 400016, P.R. China Chongqing Health Service Center, Chongqing 400000, P.R. China Key Laboratory of Birth Defects and Reproductive Health, Chongqing Population and Family Planning of Chongqing Science and Technology Research Institute, Chongqing 400000, P.R. China AU - Yang,Huan AU - Wang,Shuang AU - Kang,Yu-Jun AU - Wang,Chuan AU - Xu,Yongzhu AU - Zhang,Yi AU - Jiang,Zheng DA - 2018/07/01 DO - 10.3892/or.2018.6412 EP - 271 IS - 1 JO - Oncol Rep KW - lncRNA SNHG1 colon cancer proliferation invasion Wnt/β-catenin signaling pathway PY - 2018 SN - 1021-335X 1791-2431 SP - 261 ST - Long non-coding RNA SNHG1 predicts a poor prognosis and promotes colon cancer tumorigenesis T2 - Oncology Reports TI - Long non-coding RNA SNHG1 predicts a poor prognosis and promotes colon cancer tumorigenesis UR - https://doi.org/10.3892/or.2018.6412 VL - 40 ER -