TY - JOUR AB - Type 1 insulin‑like growth factor receptor (IGF‑IR) signaling is considered to serve a key role in the development of cancer. However, the effects of IGF‑IR on the malignant characteristics of myelodysplastic syndrome (MDS) clonal cells remains to be determined. In the present study it was demonstrated that knockdown of IGF‑IR reduced the proliferation and increased the apoptosis of MDS/leukemia cells. Integrated analysis of gene expression profiles using bioinformatics identified the MAPK signaling pathway as a critical downstream factor of IGF‑IR, and this was confirmed in vitro using western blotting which revealed that IGF‑IR knockdown significantly increased the expression of activated MAPK. Furthermore, IGF‑IR signaling was inhibited to investigate the potential of IGF‑IR as a therapeutic target of MDS. The results revealed that the IGF‑IR inhibitor picropodophyllin (PPP) inhibited cell proliferation, promoted cell apoptosis and arrested the cell cycle at the G2/M phase in MDS/leukemia cells. Similar to the effects of IGF‑IR knockdown, PPP treatment also increased MAPK signaling in vitro. In conclusion, IGF‑IR may serve as a potential therapeutic target of MDS. AD - Department of Hematology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China AU - He,Qi AU - Zheng,Qingqing AU - Xu,Feng AU - Shi,Wenhui AU - Guo,Juan AU - Zhang,Zheng AU - Zhao,Sida AU - Li,Xiao AU - Chang,Chunkang DA - 2020/09/01 DO - 10.3892/or.2020.7652 EP - 1104 IS - 3 JO - Oncol Rep KW - myelodysplastic syndrome IGF‑IR clonal cell proliferation MAPK PY - 2020 SN - 1021-335X 1791-2431 SP - 1094 ST - IGF‑IR promotes clonal cell proliferation in myelodysplastic syndromes via inhibition of the MAPK pathway T2 - Oncology Reports TI - IGF‑IR promotes clonal cell proliferation in myelodysplastic syndromes via inhibition of the MAPK pathway UR - https://doi.org/10.3892/or.2020.7652 VL - 44 ER -