TY - JOUR AB - Long non‑coding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titin‑antisense RNA1 (TTN‑AS1), miR‑27b‑3p and Runt‑related transcription factor 1 (RUNX1) expression in glioma tissues and cell lines was estimated by RT‑qPCR. Si‑TTN‑AS1 was transfected into glioma cell lines (U251 and LN229), and CCK‑8 assay, flow cytometry, wound healing and Transwell assays were applied to estimate the function of TTN‑AS1 in glioma cells. miR‑27b‑3p inhibitor was used to explore the mechanisms. The results revealed that TTN‑AS1 was highly expressed in glioma specimens and cell lines. Downregulation of TTN‑AS1 inhibited the proliferation, migration and invasion of the glioma cells, as well as increased the rate of apoptosis. In vivo, the tumor growth was also inhibited by TTN‑AS1 depletion in nude mice. Furthermore, we revealed that TTN‑AS1 exerted oncogenic effects via sponging miR‑27b‑3p and thereby positively regulating RUNX1 expression. In conclusion, the present study supported that TTN‑AS1 acts as an oncogene in glioma by targeting miR‑27b‑3p to release RUNX1. This finding may contribute to gene therapy of glioma. AD - Department of Neurosurgery, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China Department of Oncology, The Traditional Chinese Medicine Hospital of Jingmen, Jingmen, Hubei 448000, P.R. China Department of Neurosurgery, Longhai Hospital of Kaifeng City, Kaifeng, Henan 475000, P.R. China Department of Orthopaedics, The Third People's Hospital of Anyang, Anyang, Henan 455000, P.R. China Department of Neurosurgery, Kaifeng Central Hospital, Kaifeng, Henan 475000, P.R. China Department of Intervention Therapy, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China Department of Neurosurgery, The People's Hospital of Pingyu, Pingyu, Henan 463400, P.R. China AU - Chang,Keliang AU - Wang,Genwei AU - Lou,Jinfeng AU - Hao,Sha AU - Lv,Ranbo AU - Duan,Desheng AU - Zhang,Wanhong AU - Guo,Yingchang AU - Wang,Pengfei DA - 2020/09/01 DO - 10.3892/or.2020.7684 EP - 1074 IS - 3 JO - Oncol Rep KW - glioma TTN‑AS1 miR‑27b‑3p RUNX1 PY - 2020 SN - 1021-335X 1791-2431 SP - 1064 ST - lncRNA TTN‑AS1 upregulates RUNX1 to enhance glioma progression via sponging miR‑27b‑3p T2 - Oncology Reports TI - lncRNA TTN‑AS1 upregulates RUNX1 to enhance glioma progression via sponging miR‑27b‑3p UR - https://doi.org/10.3892/or.2020.7684 VL - 44 ER -