TY - JOUR AB - The aim of the present study was to explore the antitumor effects of sinoporphyrin sodium (DVDMS)‑mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms. The uptake of DVDMS by U‑118 MG cells was detected by flow cytometry (FCM). A 630‑nm semiconductor laser and 1‑MHz ultrasound were used to perform PDT and SDT, respectively. Cell proliferation and apoptosis were evaluated using the Cell Counting Kit‑8 assay, FCM and Hoechst 33258 staining, respectively. Western blot analysis was used to detect protein expression and phosphorylation levels. BALB/c nude mice were used to establish a xenograft model of U‑118 MG cells. DVDMS was injected intravenously and PDT and SDT were performed 24 h later. An in vivo imaging system was used to evaluate the fluorescence of DVDMS, to measure tumor sizes, and to evaluate the therapeutic effects. The uptake of DVDMS by U‑118 MG cells was optimal after 4 h. PDT and SDT following DVDMS injection significantly inhibited the proliferation and increased apoptosis of glioma cells in vitro (P<0.05, P<0.01) respectively. In vivo, the fluorescence intensity of DVDMS was lower in the PDT and SDT groups compared with the DVDMS group, while tumor cell proliferation and weight were lower in the PDT and SDT groups than in the control group (P<0.05, P<0.01). However, there was no significant difference when laser, ultrasound or DVDMS were applied individually, compared with the control group. Hematoxylin and eosin staining suggested that both PDT and SDT induced significant apoptosis and vascular obstruction in cancer tissues. DVDMS‑mediated PDT and SDT inhibited the expression levels of proliferating cell nuclear antigen (PCNA) and Bcl‑xL, increased cleaved ‑caspase 3 levels, and decreased the protein phosphorylation of the PI3K/AKT/mTOR signaling pathway. Changes in the expression of PCNA, and Bcl‑xL and in the levels of cleaved‑caspase 3 were partly reversed by N‑acetyl‑L‑cysteine, a reactive oxygen species (ROS) scavenger. Similar results were obtained with FCM. DVDMS‑mediated PDT and SDT inhibited glioma cell proliferation and induced cell apoptosis in vitro and in vivo, potentially by increasing the generation of ROS and affecting protein expression and phosphorylation levels. AD - School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, Guangdong 518055, P.R. China Science and Education Department, Shenzhen Samii Medical Center, Shenzhen, Guangdong 518118, P.R. China Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China Henan Key Laboratory of Medical Tissue Regeneration, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453002, P.R. China Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R. China AU - An,Ya‑Wen AU - Liu,Han‑Qing AU - Zhou,Zi‑Qian AU - Wang,Jian‑Chun AU - Jiang,Guang‑Yu AU - Li,Zhi‑Wen AU - Wang,Feng AU - Jin,Hong‑Tao DA - 2020/10/01 DO - 10.3892/or.2020.7695 EP - 1604 IS - 4 JO - Oncol Rep KW - sinoporphyrin sodium photodynamic therapy sonodynamic therapy glioma protein expression and phosphorylation PY - 2020 SN - 1021-335X 1791-2431 SP - 1596 ST - Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma T2 - Oncology Reports TI - Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma UR - https://doi.org/10.3892/or.2020.7695 VL - 44 ER -