TY - JOUR AB - Tricellulin is a tight‑junction transmembrane protein that regulates cell‑cell interactions. Altered tricellulin expression could promote tumor cell invasions and metastasis in human cancers. The present study assessed tricellulin expression in colorectal cancer tissues for any association with clinicopathological features of colorectal cancer patients and then investigated the underlying molecular events using quantitative proteomic analysis and in vitro experiments. Tissue samples from 98 colorectal cancer patients and 15 volunteers were collected for immunohistochemistry. Colorectal cell lines were used to overexpress or knockdown tricellulin expression in various assays. The data revealed that upregulated tricellulin expression was associated with lymph node and distant metastases and poor prognosis, while tricellulin overexpression promoted colorectal cancer cell migration and invasion in vitro. In contrast, tricellulin knockdown had positive effects on the tumor cells. Furthermore, TMT‑LC‑MS/MS and bioinformatics analyses revealed that tricellulin was involved in EMT and reduction of apoptosis through the NF‑κB signaling pathway. These findings highlight for the first time the significance of tricellulin in colorectal cancer development and progression. Further study may validate tricellulin as a novel biomarker and target for colorectal cancer. AD - Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, P.R. China AU - Zhang,Jin‑Xiu AU - Qin,Meng‑Bin AU - Ye,Zhe AU - Peng,Peng AU - Li,Si‑Man AU - Song,Qian AU - Lin,Lan AU - Liu,Shi‑Quan AU - Xie,Li‑Hua AU - Zhu,Ye AU - Huang,Jie‑An DA - 2020/11/01 DO - 10.3892/or.2020.7773 EP - 2184 IS - 5 JO - Oncol Rep KW - colorectal cancer tricellulin prognosis tandem mass tag‑liquid chromatography‑mass spectrometry PY - 2020 SN - 1021-335X 1791-2431 SP - 2174 ST - Association of tricellulin expression with poor colorectal cancer prognosis and metastasis T2 - Oncology Reports TI - Association of tricellulin expression with poor colorectal cancer prognosis and metastasis UR - https://doi.org/10.3892/or.2020.7773 VL - 44 ER -