TY - JOUR AB - The epithelial cell adhesion molecule (EpCAM) is a calcium‑independent, homophilic, intercellular adhesion factor classified as a transmembrane glycoprotein. In addition to cell adhesion, EpCAM also contributes to cell signaling, differentiation, proliferation, and migration. EpCAM is an essential factor in the carcinogenesis of numerous human cancers. In the present study, we developed and validated an anti‑EpCAM monoclonal antibody (mAb), EpMab‑16 (IgG2a, kappa), by immunizing mice with EpCAM‑overexpressing CHO‑K1 cells. EpMab‑16 specifically reacted with endogenous EpCAM in oral squamous cell carcinoma (OSCC) cell lines in flow cytometry and Western blot analyses. It exhibited a plasma membrane‑like stain pattern in OSCC tissues upon immunohistochemical analysis. The KD for EpMab‑16 in SAS and HSC‑2 OSCC cells were assessed via flow cytometry at 1.1x10‑8 and 1.9x10‑8 M, respectively, suggesting moderate binding affinity of EpMab‑16 for EpCAM. We then assessed whether the EpMab‑16 induced antibody‑dependent cellular cytotoxicity (ADCC) and complement‑dependent cytotoxicity (CDC) against OSCC cell lines, and antitumor capacity in a murine xenograft model. In vitro experiments revealed strong ADCC and CDC inducement against OSCC cells treated with EpMab‑16. In vivo experiments on OSCC xenografts revealed that EpMab‑16 treatment significantly reduced tumor growth compared with the control mouse IgG. These data indicated that EpMab‑16 could be a promising treatment option for EpCAM‑expressing OSCCs. AD - Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Aoba‑ku, Sendai, Miyagi 980‑8575, Japan Institute of Microbial Chemistry (BIKAKEN), Numazu, Microbial Chemistry Research Foundation, Numazu‑shi, Shizuoka 410‑0301, Japan Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Bunkyo‑ku, Tokyo 113‑8510, Japan AU - Kaneko,Mika,K. AU - Ohishi,Tomokazu AU - Takei,Junko AU - Sano,Masato AU - Nakamura,Takuro AU - Hosono,Hideki AU - Yanaka,Miyuki AU - Asano,Teizo AU - Sayama,Yusuke AU - Harada,Hiroyuki AU - Kawada,Manabu AU - Kato,Yukinari DA - 2020/12/01 DO - 10.3892/or.2020.7808 EP - 2526 IS - 6 JO - Oncol Rep KW - EpCAM monoclonal antibody ADCC CDC antitumor activity oral cancer PY - 2020 SN - 1021-335X 1791-2431 SP - 2517 ST - Anti‑EpCAM monoclonal antibody exerts antitumor activity against oral squamous cell carcinomas T2 - Oncology Reports TI - Anti‑EpCAM monoclonal antibody exerts antitumor activity against oral squamous cell carcinomas UR - https://doi.org/10.3892/or.2020.7808 VL - 44 ER -