TY - JOUR AB - To date, there is no effective therapy available for the treatment of castration‑resistant prostate cancer (CRPC), and patients generally succumb to the disease within 2 to 4 years. In the progression of CRPC, androgen receptor (AR) and its splice variants play critical roles. Hence, it is necessary to develop a drug to inhibit the expression and activity of the full‑length and splice variants of AR for the treatment of CRPC. Erastin, as the first discovered drug to induce ferroptosis, has been studied in various types of cancer. However, there are few studies focusing on the relationship between erastin and AR. In the present study, western blotting, and sulforhodamine B cell viability, glutathione, lipid peroxidation and reactive oxygen species assays were performed to verify the ferroptosis of CRPC cells; reverse transcription‑quantitative polymerase chain reaction, dual‑luciferase reporter, and lentiviral packaging and lentivirus‑infected cell assays were employed to evaluate how erastin affects AR. A mouse xenograft assay was used to determine the underlying mechanism in vivo. Erastin, as a classical inducer of ferroptosis, can suppress the transcriptional activities of both the full‑length and splice variants in AR models in vitro and in vivo. In addition, when erastin was used for CRPC treatment combined with docetaxel, the growth inhibitory efficacy of docetaxel was found to be enhanced. Thus, these findings indicated that ferroptosis inducer erastin has potential in the treatment of CRPC via targeting AR. AD - Department of Recovery, Nursing School, Jilin University, Changchun, Jilin 130021, P.R. China Department of Breast Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China Department of National Engineering Laboratory for AIDS Vaccine, College of Life Sciences, Jilin University, Changchun, Jilin 130021, P.R. China Department of Analysis and Testing Center, Jilin Academy of Environmental Sciences, Changchun, Jilin 130021, P.R. China Department of Gynaecology and Obstetrics, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China AU - Yang,Yanrong AU - Liu,Taiyuan AU - Hu,Cheng AU - Xia,Hongyan AU - Liu,Wei AU - Chen,Junyu AU - Wu,Shan AU - Jiang,Yu AU - Xu,Yang AU - Liu,Wanxia AU - Zhao,Lijing DA - 2021/04/01 DO - 10.3892/or.2021.7976 IS - 4 JO - Oncol Rep KW - castration‑resistant prostate cancer erastin androgen receptor splice variants PY - 2021 SN - 1021-335X 1791-2431 SP - 25 ST - Ferroptosis inducer erastin downregulates androgen receptor and its splice variants in castration‑resistant prostate cancer T2 - Oncology Reports TI - Ferroptosis inducer erastin downregulates androgen receptor and its splice variants in castration‑resistant prostate cancer UR - https://doi.org/10.3892/or.2021.7976 VL - 45 ER -