TY - JOUR
AB - Triple‑negative breast cancer (TNBC) behaves aggressively in the invasive and metastatic states. Our research group recently developed a novel curcumin derivative, (1E,3Z,6E)-3-hydroxy-5-oxohepta-1,3,6-triene-1,7-diyl)bis(2‑methoxy-4,1‑phenylene)bis(3-hydroxy2-hydroxymethyl)-2‑methyl propanoate (MTH‑3), and previous studies showed that MTH‑3 inhibits TNBC proliferation and induces apoptosis in vitro and in vivo with a superior bioavailability and absorption than curcumin. In the present study, the effects of MTH‑3 on TNBC cell invasion were examined using various assays and gelatin zymography, and western blot analysis. Treatment with MTH‑3 inhibited MDA‑MB‑231 cell invasion and migration, as shown by Transwell assay, 3D spheroid invasion assay, and wound healing assay. The results of the gelatin zymography experiments revealed that MTH‑3 decreased matrix metalloproteinase‑9 activity. The potential signaling pathways were revealed by next‑generation sequencing analysis, antibody microarray analysis and western blot analysis. In conclusion, the results of the present study show that, MTH‑3 inhibited tumor cell invasion through the MAPK/ERK/AKT signaling pathway and cell cycle regulatory cascade, providing significant information about the potential molecular mechanisms of the effects of MTH‑3 on TNBC.
AD - Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C.
Human Genetic Center, China Medical University, Taichung 40402, Taiwan, R.O.C.
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan, R.O.C.
Chinese Medicinal Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan, R.O.C.
Department of Sport Performance, National Taiwan University of Sport, Taichung 40402, Taiwan, R.O.C.
AU - Chiu,Yu-Jen
AU - Tsai,Fuu-Jen
AU - Bau,Da-Tian
AU - Chang,Ling-Chu
AU - Hsieh,Min-Tsang
AU - Lu,Chi-Cheng
AU - Kuo,Sheng-Chu
AU - Yang,Jai-Sing
AU - Chiu,Yu-Jen
AU - Tsai,Fuu-Jen
AU - Bau,Da-Tian
AU - Chang,Ling-Chu
AU - Hsieh,Min-Tsang
AU - Lu,Chi-Cheng
AU - Kuo,Sheng-Chu
AU - Yang,Jai-Sing
DA - 2021/07/01
DO - 10.3892/or.2021.8084
IS - 1
JO - Oncol Rep
KW - curcuminoid derivative
triple‑negative breast cancer (TNBC)
anti‑metastasis
MTH‑3
next generation sequencing analysis
PY - 2021
SN - 1021-335X
1791-2431
SP - 133
ST - Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells
T2 - Oncology Reports
TI - Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells
UR - https://doi.org/10.3892/or.2021.8084
VL - 46
ER -