TY - JOUR
AB - Erythropoietin‑producing hepatocellular receptors (Ephs) comprise the largest subfamily of receptor tyrosine kinases and have been reported to be involved in a variety of biological cellular processes, including tumorigenesis and cancer progression. The present study aimed to determine the expression levels and clinicopathological significance of EphA8 in breast cancer (BC) using immunohistochemistry analysis of tissue microarrays. The results of the present study revealed that EphA8 expression levels were upregulated in BC tissue and were associated with tumor size and TNM stage. In addition, upregulated expression levels of EphA8 were identified to be a poor prognostic biomarker for patients with BC. The knockdown of EphA8 expression using short hairpin RNA resulted in increased levels of apoptosis as well as decreased proliferation, migration and invasion of BC cells both in vivo and in vitro. The knockdown of EphA8 also decreased the phosphorylation of AKT, which was accompanied by downregulation of Bcl‑2 expression levels and upregulation of p53, Caspase‑3 and Bax expression levels. Moreover, knockdown of EphA8 expression increased the chemosensitivity of BC cells to paclitaxel. In conclusion, the results of the present study indicated that EphA8 may be a useful prognostic marker in BC and that knockdown of EphA8 may represent a novel strategy in adjuvant chemotherapy for the treatment of BC.
AD - Department of Clinical Biobank, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
AU - Wang,Gui-Hua
AU - Ni,Kan
AU - Gu,Changjiang
AU - Huang,Jianfei
AU - Chen,Jing
AU - Wang,Xu-Dong
AU - Ni,Qichao
DA - 2021/08/01
DO - 10.3892/or.2021.8134
IS - 2
JO - Oncol Rep
KW - EphA8
prognosis
biomarker
AKT
PY - 2021
SN - 1021-335X
1791-2431
SP - 183
ST - EphA8 inhibits cell apoptosis via AKT signaling and is associated with poor prognosis in breast cancer
T2 - Oncology Reports
TI - EphA8 inhibits cell apoptosis via AKT signaling and is associated with poor prognosis in breast cancer
UR - https://doi.org/10.3892/or.2021.8134
VL - 46
ER -