TY - JOUR
AB - The aim of the present study was to investigate the expression of ribosome assembly factor partner of NOB1 homolog (PNO1) and its association with the progression of breast cancer (BC) in patients, as well as its biological function and underlying mechanism of action in BC cells. Bioinformatics and immunohistochemical analyses revealed that PNO1 expression was significantly increased in BC tissues and its high mRNA expression was associated with shorter overall survival (OS) and relapse‑free survival (RFS) of patients with BC, as well as multiple clinical characteristics (including advanced stage of NPI and SBR, etc.) of patients with BC. Biological functional studies revealed that transduction of lentivirus encoding sh‑PNO1 significantly downregulated PNO1 expression, reduced cell confluency and the number of BC cells in vitro and inhibited tumor growth in vivo. Moreover, PNO1 knockdown decreased the cell viability and arrested cell cycle progression at the G2/M phase, as well as downregulated cyclin B1 (CCNB1) and cyclin‑dependent kinase 1 (CDK1) protein expression in BC cells. Correlation analysis demonstrated that PNO1 expression was positively correlated with both CDK1 and CCNB1 expression in BC samples. Collectively, PNO1 was upregulated in BC and associated with BC patient survival, and PNO1 knockdown suppressed tumor growth in vitro and in vivo. In addition, positive regulation of CCNB1 and CDK1 may be one of the underlying mechanisms.
AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
Department of Pediatrics, Case Western Reserve University School of Medicine, Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA
Department of Oncology, No. 900 Hospital of The Joint Logistic Support Force, Fuzhou, Fujian 350025, P.R. China
AU - Li,Jie
AU - Liu,Liya
AU - Chen,Youqin
AU - Wu,Meizhu
AU - Lin,Xiaoying
AU - Shen,Zhiqing
AU - Cheng,Ying
AU - Chen,Xiaoping
AU - Weygant,Nathaniel
AU - Wu,Xiangyan
AU - Wei,Lihui
AU - Sferra,Thomas,J.
AU - Han,Yuying
AU - Chen,Xi
AU - Shen,Aling
AU - Shen,Aling
AU - Peng,Jun
DA - 2022/06/01
DO - 10.3892/or.2022.8319
IS - 6
JO - Oncol Rep
KW - partner of NOB1 homolog
breast cancer
survival
tumor growth
cell cycle
PY - 2022
SN - 1021-335X
1791-2431
SP - 108
ST - Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer
T2 - Oncology Reports
TI - Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer
UR - https://doi.org/10.3892/or.2022.8319
VL - 47
ER -