TY - JOUR AB - Programmed cell death 4 (PDCD4) is regarded as an important tumor suppressor that is lowly expressed or deleted in numerous human types of cancer, including ovarian and endometrial cancer. Tripartite motif‑containing 27 (TRIM27) is closely related to the occurrence and development of tumors and is highly expressed in numerous types of cancer such as ovarian and endometrial cancer. PDCD4 can be degraded through ubiquitination, while TRIM27 has the E3 ubiquitin ligase activity. However, whether TRIM27 may regulate the expression of PDCD4 by ubiquitination effect remains unclear. In the present study, the expression of PDCD4 and TRIM27 in different ovarian and endometrial cancer cell lines was detected by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting and immunocytochemistry. The impact of TRIM27 overexpression and knockdown on PDCD4 expression and the effective mechanism of TRIM27 regulating PDCD4 expression were also investigated in vitro by RT‑qPCR, western blotting, co‑immunoprecipitation assay, Transwell migration and Matrigel invasion assays. The results showed that the expression of TRIM27 and PDCD4 had a negative association at the protein level, and the distribution of TRIM27 and PDCD4 proteins had a phenomenon of co‑localization in different ovarian and endometrial cancer cell lines. TRIM27 promoted the degradation of PDCD4 through the ubiquitin‑proteasome pathway. To sum up, TRIM27 could increase the migration and invasion of ovarian and endometrial cancer cells by promoting the ubiquitination and degradation of PDCD4. The present findings may provide a new target for the treatment of ovarian and endometrial cancer. AD - Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China Department of Gynecology and Obstetrics, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250013, P.R. China Department of Gynecology and Obstetrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China AU - Yu,Huayun AU - Wan,Lu AU - Tang,Zhongyun AU - Yao,Chenchen AU - Zhang,Derui AU - Jiang,Mengmeng AU - Wang,Chongli AU - Liu,Yuqiu AU - Xue,Chenyue AU - Wang,Xishuang AU - Shi,Yongyu AU - Zhang,Lining AU - Wang,Xiaoyan AU - Wei,Zengtao AU - Yu,Huayun AU - Wan,Lu AU - Tang,Zhongyun AU - Yao,Chenchen AU - Zhang,Derui AU - Jiang,Mengmeng AU - Wang,Chongli AU - Liu,Yuqiu AU - Xue,Chenyue AU - Wang,Xishuang AU - Shi,Yongyu AU - Zhang,Lining AU - Wang,Xiaoyan AU - Wei,Zengtao DA - 2022/07/01 DO - 10.3892/or.2022.8331 IS - 1 JO - Oncol Rep KW - tripartite motif‑containing 27 programmed cell death 4 ubiquitin‑proteasome pathway ovarian and endometrial cancer PY - 2022 SN - 1021-335X 1791-2431 SP - 120 ST - TRIM27 regulates the expression of PDCD4 by the ubiquitin‑proteasome pathway in ovarian and endometrial cancer cells T2 - Oncology Reports TI - TRIM27 regulates the expression of PDCD4 by the ubiquitin‑proteasome pathway in ovarian and endometrial cancer cells UR - https://doi.org/10.3892/or.2022.8331 VL - 48 ER -