TY - JOUR AB - Cervical cancer is a common malignant tumor in females. Orthodenticle homolog 1 (OTX1) serves a key role in the occurrence and progression of tumors. The present study aimed to investigate the role and potential mechanism of OTX1 in cervical cancer. OTX1 expression was analyzed by western blotting, reverse transcription‑quantitative PCR and immunohistochemistry. MTT assay was performed to assess cell viability. EdU and colony formation assay were used to measure cell proliferation. Wound healing and Transwell assays were performed to measure cell migration and invasion. Western blot assay was performed for the assessment of protein expression. Gene set enrichment analysis (GSEA) was performed to analyze signaling pathways regulated by OTX1. Co‑Immunoprecipitation assay was performed to confirm the interaction between OTX1 and Wnt9b. In cervical cancer tissue and cells, OTX1 was significantly upregulated. OTX1 overexpression promoted proliferation, migration and invasion of cervical cancer cells. OTX1 silencing significantly decreased cell proliferation, migration and invasion of cervical cancer. GSEA showed that OTX1 activated the Wnt signaling pathway. OTX1 silencing inhibited the increased levels of adenomatous polyposis coli (APC), glycogen synthase kinase (GSK)‑3β and axis inhibition protein (AXIN)2 and decreased levels of Wnt9b and β‑catenin. OTX1 overexpression decreased the levels of APC, GSK‑3β and AXIN2 and increased levels of Wnt9b and β‑catenin. However, XAV939 (a Wnt signaling inhibitor) and β‑catenin silencing partly eliminated the effect of OTX1 overexpression on cervical cancer cells. OTX1 promoted the progression of cervical cancer by activating the Wnt signaling pathway. AD - Department of Gynecology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Hongshan, Wuhan, Hubei 430070, P.R. China AU - Zhou,Limin AU - Li,Hongying AU - Zhang,Dunlan AU - Chen,Lu AU - Dong,Hong AU - Yuan,Yuqin AU - Wang,Tinghui DA - 2022/11/01 DO - 10.3892/or.2022.8419 IS - 5 JO - Oncol Rep KW - cervical cancer orthodenticle homolog 1 proliferation Wnt signaling pathway PY - 2022 SN - 1021-335X 1791-2431 SP - 204 ST - OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway T2 - Oncology Reports TI - OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway UR - https://doi.org/10.3892/or.2022.8419 VL - 48 ER -