TY - JOUR AB - Diffuse large B‑cell lymphoma (DLBCL) is one of the most common types of lymphoma. Calponin 3 (CNN3) is a thin filament‑associated protein previously known to regulate smooth muscle contraction. Recent evidence illustrates its involvement in carcinogenesis; however, its roles in DLBCL remain unknown. CNN3 was found to be highly expressed in DLBCL specimens according to the online Gene Expression Profiling Interactive Analysis data. The aim of the present study was to investigate the roles of CNN3 in the progression of DLBCL. In vitro, the ectopic expression of CNN3 promoted the proliferation and G1/S transition of DLBCL cells, while its silencing led to opposite alterations. A similar tumor‑promoting role of CNN3 was also demonstrated by injecting nude mice with DLBCL cells over‑ or underexpressing CNN3. The results of dual‑luciferase reporter and chromatin immunoprecipitation assays revealed that forkhead box O3 (FOXO3), a known tumor suppressor in DLBCL, bound to the CNN3 promoter at ‑1955/‑1948 and ‑1190/‑1183, and suppressed the transcription of CNN3. The alterations induced by FOXO3 were partly blocked by CNN3 overexpression. On the whole, the present study demonstrates that CNN3, whose transcriptional activity is negatively regulated by FOXO3, contributes to the malignant behavior of DLBCL cells. The findings of the present study may provide novel diagnostic or therapeutic insight for DLBCL in clinical practice. AD - Department of Hematology and Breast Cancer, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital and Institute), Shenyang, Liaoning 110042, P.R. China Department of Pathology, Shenyang Medical College, Shenyang, Liaoning 110034, P.R. China Department of Scientific Research and Academic, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital and Institute), Shenyang, Liaoning 110042, P.R. China AU - Xing,Xiaojing AU - Liu,Meichen AU - Wang,Xuguang AU - Guo,Qianxue AU - Wang,Hongyue DA - 2023/02/01 DO - 10.3892/or.2023.8483 IS - 2 JO - Oncol Rep KW - diffuse large B‑cell lymphoma calponin 3 forkhead box O3 proliferation apoptosis PY - 2023 SN - 1021-335X 1791-2431 SP - 46 ST - Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells T2 - Oncology Reports TI - Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells UR - https://doi.org/10.3892/or.2023.8483 VL - 49 ER -