TY - JOUR AB - To determine the clinical significance of gene promoter methylation in esophageal squamous cell carcinoma (ESCC), we examined the promoter methylation status of genes showing elevated expression, as determined by DNA microarray-based transcriptomic analysis, in three ESCC cell lines (TE-1, TE-2, TE-10) after 5-aza-2'-deoxycytidine (DAC) treatment. We observed a high degree of DNA methylation within the promoter regions of three genes, namely cathepsin L2 (CTSL2), normal mucosa of esophagus specific 1 (NMES1), and fatty acid binding protein 5 (FABP5). Overexpression of NMES1 in ESCC cell lines increased cell motility. Down-regulation of NMES1 might play an important role in the cell motility of ESCC or be a potent marker of malignancy. AD - Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. araim-cib@umin.ac.jp null AU - Arai,Makoto AU - Imazeki,Fumio AU - Sakai,Yuzo AU - Mikata,Rintaro AU - Tada,Motohisa AU - Seki,Naohiko AU - Shimada,Hideaki AU - Ochiai,Takenori AU - Yokosuka,Osamu DA - 2008/08/01 DO - 10.3892/or_00000022 EP - 412 IS - 2 JO - Oncol Rep PY - 2008 SN - 1021-335X 1791-2431 SP - 405 ST - Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma T2 - Oncology Reports TI - Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2'-deoxycytidine, in esophageal squamous cell carcinoma UR - https://doi.org/10.3892/or_00000022 VL - 20 ER -