TY - JOUR AB - The effect of genistein, a protein tyrosine kinase and topoisomerase II inhibitor, on the DNA synthesis rate was studied in 21 human glioma specimens obtained at routine craniotomies for tumor resection. Ongoing DNA synthesis rate was determined by using a method based on the generation of tissue mini-units immediately after tumor resection and short incubation time (0-120 min) with [methyl-3H]-thymidine. A 9-77% inhibition of DNA synthesis rate by 100 µM genistein was observed in 18/21 of the glioma specimens. In these cases, the average percentage of inhibition was 55±20% (mean ± SD, P<0.0001, Student's t-test) and the inhibitory effect was >50% in 12/18 of the cases. In 3 cases genistein increased the DNA synthesis rate. The inhibitory effect of genistein had a short-time onset and was concentration-dependent. Additional experiments in 4 cases showed that herbimycin A had no effect on DNA synthesis rate while etoposide inhibited similarly to that of genistein. Our results suggest that the effect of genistein on DNA synthesis rate in gliomas is independent of protein kinase inhibition and probably mediated by topoisomerase II inhibition. In the RG2 model, 50 µM genistein inhibited ongoing DNA synthesis in glioma cells with little or no effect in normal tissue. The data also encourage further investigations on the therapeutic potential of genistein for gliomas. AD - Division of Neurology, Department of Clinical Neuroscience, Karolinska University Hospital Huddinge, Karolinska Institute, Stockholm, Sweden. sebastian.yakisich@ki.se or yakisich@gmail.com null AU - Yakisich,Juan,Sebastian AU - Ohlsson Lindblom,Ingrid AU - Siden,Åke AU - Cruz,Mabel,H. DA - 2009/09/01 DO - 10.3892/or_00000473 EP - 574 IS - 3 JO - Oncol Rep PY - 2009 SN - 1021-335X 1791-2431 SP - 569 ST - Rapid inhibition of ongoing DNA synthesis in human glioma tissue by genistein T2 - Oncology Reports TI - Rapid inhibition of ongoing DNA synthesis in human glioma tissue by genistein UR - https://doi.org/10.3892/or_00000473 VL - 22 ER -