Genomic analysis of invasion-metastasis-related factors in pancreatic cancer cells

  • Authors:
    • Xiaodong Tan
    • Lei Zhou
    • Wei Wang
    • Baosheng Wang
    • Hiroshi Egami
    • Hideo Baba
    • Xianwei Dai
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  • Published online on: January 1, 2010     https://doi.org/10.3892/etm_00000034
  • Pages: 211-216
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Abstract

Pancreatic cancer is known to be an extremely lethal neoplasm, one of the reasons being that pancreatic cancer itself has an extremely high potential of invasion-metastasis. In our previous study, two pancreatic cancer cell lines with a different potential for invasion-metastasis, PC-1 with a low potential and PC-1.0 with a high potential of invasion-metastasis after intrapancreatic transplantation, were established in a Syrian golden hamster. To determine the invasion-metastasis-related factors, a cDNA microarray that represented a set of 27,000 genes was hybridized with a labeled cDNA probe and screened for molecular profiling analysis. Furthermore, Gene Ontology and Pathway differential expression of candidate genes was further validated using RT-PCR. One hundred and forty-one differentially expressed genes (>3.0-fold change) were identified in the present study, including 46 up-regulated genes (e.g., nup107, tjp-2 and MMP-13) and 95 down-regulated genes (e.g., Spc21, plau and CD44) in the PC-1.0 cells. Our present results suggest that a highly organized and structured process of tumor invasion-metastasis exists in the pancreas. Analysis of gene expression profiles by cDNA microarray provides useful information for clarifying the mechanism underlying this invasion and metastasis. Furthermore, the identification of invasion-metastasis-specific genes may allow us to develop new therapeutic and diagnostic targets for the invasion-metastasis of pancreatic cancer.
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January-February 2010
Volume 1 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tan X, Zhou L, Wang W, Wang B, Egami H, Baba H and Dai X: Genomic analysis of invasion-metastasis-related factors in pancreatic cancer cells . Exp Ther Med 1: 211-216, 2010
APA
Tan, X., Zhou, L., Wang, W., Wang, B., Egami, H., Baba, H., & Dai, X. (2010). Genomic analysis of invasion-metastasis-related factors in pancreatic cancer cells . Experimental and Therapeutic Medicine, 1, 211-216. https://doi.org/10.3892/etm_00000034
MLA
Tan, X., Zhou, L., Wang, W., Wang, B., Egami, H., Baba, H., Dai, X."Genomic analysis of invasion-metastasis-related factors in pancreatic cancer cells ". Experimental and Therapeutic Medicine 1.1 (2010): 211-216.
Chicago
Tan, X., Zhou, L., Wang, W., Wang, B., Egami, H., Baba, H., Dai, X."Genomic analysis of invasion-metastasis-related factors in pancreatic cancer cells ". Experimental and Therapeutic Medicine 1, no. 1 (2010): 211-216. https://doi.org/10.3892/etm_00000034