Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs

  • Authors:
    • Haiying Yue
    • Kai Hu
    • Wenqi Liu
    • Jie Jiang
    • Yuhua Chen
    • Rensheng Wang
  • View Affiliations

  • Published online on: July 27, 2015     https://doi.org/10.3892/etm.2015.2658
  • Pages: 1437-1444
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Abstract

Radiation-induced lung injury (RILI) is a common complication associated with thoracic radiotherapy. The aim of the present study was to investigate the effects of a single 15-Gy dose of right-thoracic lung irradiation on the expression levels of matrix metalloproteinases (MMPs) and other proteins in the alveolar epithelial type II (AE2) cells of Bama minipigs. All minipigs received either right‑thoracic irradiation or sham irradiation under anesthesia, and were sacrificed at 4, 8, 12 or 24 weeks after irradiation. Collagen deposition was measured using Masson's trichrome staining. Surfactant protein A (SP‑A), transforming growth factor β1 (TGFβ1), MMP2, MMP9, vimentin and E‑cadherin protein expression levels were evaluated using western blot analysis, and the MMP2 and MMP9 gelatinase activities were tested using gelatin zymography. SP‑A and α‑smooth muscle actin (α‑SMA) co‑localization was visualized using double immunofluorescence staining. At each time‑point following irradiation, a significant increase in TGFβ1, α‑SMA, MMP2, MMP9 and vimentin protein expression levels and MMP2 and MMP9 gelatinase activity were observed in the irradiated lungs compared with the sham‑irradiated controls. By contrast, SP‑A and E‑cadherin protein expression levels decreased in a time‑dependent manner post‑irradiation. SP‑A and α‑SMA co‑localization was observed in irradiated alveolar epithelial cells. These data demonstrate that E‑cadherin, SP‑A, MMP2 and MMP9 may function as sensitive predictors of RILI. Epithelial‑mesenchymal transition (EMT) occurs in the irradiated lungs of Bama minipigs, and MMP2 and MMP9 may contribute to EMT in AE2 cells by regulating TGFβ1. Therefore, EMT may serve a crucial function in the development of RILI.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yue H, Hu K, Liu W, Jiang J, Chen Y and Wang R: Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs. Exp Ther Med 10: 1437-1444, 2015
APA
Yue, H., Hu, K., Liu, W., Jiang, J., Chen, Y., & Wang, R. (2015). Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs. Experimental and Therapeutic Medicine, 10, 1437-1444. https://doi.org/10.3892/etm.2015.2658
MLA
Yue, H., Hu, K., Liu, W., Jiang, J., Chen, Y., Wang, R."Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs". Experimental and Therapeutic Medicine 10.4 (2015): 1437-1444.
Chicago
Yue, H., Hu, K., Liu, W., Jiang, J., Chen, Y., Wang, R."Role of matrix metalloproteinases in radiation-induced lung injury in alveolar epithelial cells of Bama minipigs". Experimental and Therapeutic Medicine 10, no. 4 (2015): 1437-1444. https://doi.org/10.3892/etm.2015.2658