Open Access

Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk

  • Authors:
    • Sertaç Argun Kıvanç
    • Merih Kıvanç
    • Tülay Yiğit
  • View Affiliations

  • Published online on: July 26, 2016     https://doi.org/10.3892/etm.2016.3545
  • Pages: 1732-1740
  • Copyright: © Kıvanç et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Enterococci, which have useful biotechnological applications, produce bacteriocins, including those that exert anti-Listerial activity. The present study aimed to determine the antibiotic susceptibility patterns and antimicrobial activity of Enterococcus faecium strains isolated from human breast milk. The strains were identified using carbohydrate fermentation tests and ribotyping. Subsequently, the antibacterial activity of the isolates was investigated, and the quantities of lactic acid and hydrogen peroxide produced, and the proteolytic activity of E. faecium, were determined. In addition, biofilm formation by E. faecium strains was assessed. E. faecium strains exhibited antimicrobial activity against food‑borne and clinical bacterial isolates. Furthermore, following 24 h incubation, the tested strains exhibited resistance to a pH range of 2.0‑9.5 and tolerance of bile acid, lysozyme activity and phenol. Supernatants of the E. faecium TM13, TM15, TM17 and TM18 strains were shown to be effective against Listeria monocytogenes, and were also resistant to heat. Further studies are required in order to determine whether certain strains of E. faecium may be used for the development of novel antibacterial agents.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kıvanç SA, Kıvanç M and Yiğit T: Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Exp Ther Med 12: 1732-1740, 2016
APA
Kıvanç, S.A., Kıvanç, M., & Yiğit, T. (2016). Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Experimental and Therapeutic Medicine, 12, 1732-1740. https://doi.org/10.3892/etm.2016.3545
MLA
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12.3 (2016): 1732-1740.
Chicago
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1732-1740. https://doi.org/10.3892/etm.2016.3545