A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development

  • Authors:
    • Cuiju Wang
    • Yufei Zhao
    • Yanming Ming
    • Shengnan Zhao
    • Zhanjun Guo
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  • Published online on: July 13, 2016     https://doi.org/10.3892/etm.2016.3521
  • Pages: 1867-1872
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Abstract

MicroRNAs (miRNAs) bind to the 3'-untranslated regions (3'-UTRs) of mRNAs, affecting translation and regulating cell differentiation, tumorigenesis and apoptosis. Genetic polymorphisms in these regions in target genes are able to affect the binding affinity between miRNA and target genes, ultimately affecting the expression of individual miRNAs. In the present case‑control study, genotyping of 5 microRNA single nucleotide polymorphisms (SNPs) located at the binding site of the 3'‑UTR of RYR3 (rs1044129), C14orf101 (rs4901706), KIAA0423 (rs1053667), GOLGA7 (rs11337) and KRT81 (rs3660) genes was assessed in order to investigate its role in gastric cancer (GC). The results indicated that the rs4901706 SNP, which is located in the 3'‑UTR of C14orf101, was associated with GC development risk, as determined by χ2 analysis (relative risk, 1.630; 95% confidence interval, 1.070‑2.483; P=0.022). A Renilla/luciferase reporter assay also indicated the different binding affinity between the SNP of rs4901706 and microRNA. In conclusion, rs4901706 SNP of C14orf101 gene in the microRNA binding site may be used as a valuable biomarker when predicting GC risk.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang C, Zhao Y, Ming Y, Zhao S and Guo Z: A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development. Exp Ther Med 12: 1867-1872, 2016
APA
Wang, C., Zhao, Y., Ming, Y., Zhao, S., & Guo, Z. (2016). A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development. Experimental and Therapeutic Medicine, 12, 1867-1872. https://doi.org/10.3892/etm.2016.3521
MLA
Wang, C., Zhao, Y., Ming, Y., Zhao, S., Guo, Z."A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development". Experimental and Therapeutic Medicine 12.3 (2016): 1867-1872.
Chicago
Wang, C., Zhao, Y., Ming, Y., Zhao, S., Guo, Z."A polymorphism at the microRNA binding site in the 3'-untranslated region of C14orf101 is associated with the risk of gastric cancer development". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1867-1872. https://doi.org/10.3892/etm.2016.3521