Open Access

Expression of Toll-like receptor 4 in lungs of immune-suppressed rat with Acinetobacter baumannii infection

  • Authors:
    • Yanmei Wang
    • Xiaohong Zhang
    • Xuanlin Feng
    • Xiaoshu Liu
    • Lei Deng
    • Zong-An Liang
  • View Affiliations

  • Published online on: August 29, 2016     https://doi.org/10.3892/etm.2016.3624
  • Pages: 2599-2605
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Toll-like receptor 4 (TLR4) is involved in the regulation of host responses to Acinetobacter baumannii (A. baumannii). The aim of the present study was to examine the function of TLR4 in lung inflammation in immune‑suppressed rats with A. baumannii infection. A total of 72 Sprague‑Dawley male rats were randomly divided into the control, A. baumannii infection and immune‑suppressed infection groups. The immune‑suppressed infection group was treated with 100 mg/kg hydrocortisone by subcutaneous injection every other day for 2 weeks prior to A. baumannii infection. Lung tissue was obtained on the 3rd and 7th day after tracheal inoculation with A. baumannii. The expression of TLR4 in bronchial and alveolar epithelial cells, and alveolar macrophage was examined using immunohistochemistry. The levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α in bronchoalveolar lavage fluid were detected using ELISA. The results showed that in the control group, the expression of TLR4 was upregulated in the bronchial and alveolar epithelial, and alveolar macrophages, and the levels of IL-6 and TNF-α were increased in the early phase of A. baumannii infection. On the 7th day, no significant difference in the levels of IL-6 and TNF-α was observed between the A. baumannii infection and control groups. Conversely, the expression of TLR4 was downregulated in the immune-suppressed group, and the levels of IL-6 and TNF-α were reduced on the 3rd day after infection. In the subsequent observation period, the expression of TLR4 was upregulated and the levels of IL-6 and TNF-α were increased. In conclusion, the results show a critical role of TLR4 in mediating effective immune response in the lung of rat with A. baumannii infection.
View Figures
View References

Related Articles

Journal Cover

October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Zhang X, Feng X, Liu X, Deng L and Liang Z: Expression of Toll-like receptor 4 in lungs of immune-suppressed rat with Acinetobacter baumannii infection. Exp Ther Med 12: 2599-2605, 2016
APA
Wang, Y., Zhang, X., Feng, X., Liu, X., Deng, L., & Liang, Z. (2016). Expression of Toll-like receptor 4 in lungs of immune-suppressed rat with Acinetobacter baumannii infection. Experimental and Therapeutic Medicine, 12, 2599-2605. https://doi.org/10.3892/etm.2016.3624
MLA
Wang, Y., Zhang, X., Feng, X., Liu, X., Deng, L., Liang, Z."Expression of Toll-like receptor 4 in lungs of immune-suppressed rat with Acinetobacter baumannii infection". Experimental and Therapeutic Medicine 12.4 (2016): 2599-2605.
Chicago
Wang, Y., Zhang, X., Feng, X., Liu, X., Deng, L., Liang, Z."Expression of Toll-like receptor 4 in lungs of immune-suppressed rat with Acinetobacter baumannii infection". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2599-2605. https://doi.org/10.3892/etm.2016.3624