Open Access

Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats

  • Authors:
    • Hengrui Liu
    • Yingquan Xiong
    • Xiaofeng Zhu
    • Han Gao
    • Sujuan Yin
    • Jiefang Wang
    • Guangming Chen
    • Chaopeng Wang
    • Lu Xiang
    • Panpan Wang
    • Ji Fang
    • Ronghua Zhang
    • Li Yang
  • View Affiliations

  • Published online on: February 15, 2017     https://doi.org/10.3892/etm.2017.4128
  • Pages: 1360-1368
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Icariin (ICA) is a pharmacologically active flavonoid glycoside that shows promise in the treatment and prevention of osteoporosis (OP). However, the mechanisms underlying the anti‑osteoporotic effects of ICA remain largely unclear. The present study used quantitative polymerase chain reaction, western blot and immunohistochemical analysis to examine the effects of ICA on several key targets in the Notch signaling pathway in bone tissue in ovariectomized rats. It was observed that ICA has a pronounced beneficial effect on OP rats and inhibits the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα) and fatty acid‑binding protein 4 (FABP4) mRNA. In addition, it was identified that ICA downregulates the expression of notch1 intracellular domain (N1ICD) and Jagged1 proteins in bone tissue, and suppresses the effect of N1ICD on Notch2 mRNA expression. It is proposed that ICA inhibits the differentiation of mesenchymal stem cells into adipocytes by inhibiting the expression of PPARγ, C/EBPα and FABP4 mRNA via the Notch signaling pathway. In addition, it is proposed that ICA inhibits the expression of Notch2 mRNA by suppressing the effect of N1ICD. In conclusion, the results provide further mechanistic evidence for the clinical efficacy of ICA in the treatment of OP.
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April-2017
Volume 13 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu H, Xiong Y, Zhu X, Gao H, Yin S, Wang J, Chen G, Wang C, Xiang L, Wang P, Wang P, et al: Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats. Exp Ther Med 13: 1360-1368, 2017
APA
Liu, H., Xiong, Y., Zhu, X., Gao, H., Yin, S., Wang, J. ... Yang, L. (2017). Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats. Experimental and Therapeutic Medicine, 13, 1360-1368. https://doi.org/10.3892/etm.2017.4128
MLA
Liu, H., Xiong, Y., Zhu, X., Gao, H., Yin, S., Wang, J., Chen, G., Wang, C., Xiang, L., Wang, P., Fang, J., Zhang, R., Yang, L."Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats". Experimental and Therapeutic Medicine 13.4 (2017): 1360-1368.
Chicago
Liu, H., Xiong, Y., Zhu, X., Gao, H., Yin, S., Wang, J., Chen, G., Wang, C., Xiang, L., Wang, P., Fang, J., Zhang, R., Yang, L."Icariin improves osteoporosis, inhibits the expression of PPARγ, C/EBPα, FABP4 mRNA, N1ICD and jagged1 proteins, and increases Notch2 mRNA in ovariectomized rats". Experimental and Therapeutic Medicine 13, no. 4 (2017): 1360-1368. https://doi.org/10.3892/etm.2017.4128