Protective role of α-lipoic acid in hyperuricemia-induced endothelial dysfunction

  • Authors:
    • Hui Zou
    • Huan Wang
    • Tongmei Liu
    • Xiaojie Li
    • Xiaoduo Zhu
    • Zheng Wang
  • View Affiliations

  • Published online on: April 18, 2017     https://doi.org/10.3892/etm.2017.4345
  • Pages: 3047-3054
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Abstract

The aim of the current study was to determine the effects of α‑lipoic acid (LA) on hyperuricemia and endothelial dysfunction, and to uncover the underlying mechanism of its action. A hyperuricemic rat model was established by administration of uric acid (UA) and the rats were orally fed with 2 g/kg/day LA or phosphate‑buffered saline. Primary rat aortic endothelial cells were subsequently isolated, and a cell viability assay, apoptosis assay, enzyme nitric oxide synthase (eNOS) activity assay and mitochondrial function assay were all performed. For the in vitro study, human umbilical vein endothelial cells were used and western blotting was performed to assess Akt signaling activity. The results of the current study indicated that LA inhibited apoptosis, enhanced eNOS activity and production of nitric oxide (NO), and rescued mitochondrial mass and function in uric acid (UA)‑treated endothelial cells. LA activated Akt signaling and inhibition of Akt signaling abolished the effects of LA on cell viability, NO production, ROS production and ATP levels in UA‑treated endothelial cells. Therefore, the current study demonstrated that LA attenuated oxidant stress and inhibited apoptosis in UA‑treated endothelial cells by activating Akt signaling. The results indicate that LA may serve as a therapeutic approach to treat hyperuricemia-induced endothelial dysfunction.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zou H, Wang H, Liu T, Li X, Zhu X and Wang Z: Protective role of α-lipoic acid in hyperuricemia-induced endothelial dysfunction. Exp Ther Med 13: 3047-3054, 2017
APA
Zou, H., Wang, H., Liu, T., Li, X., Zhu, X., & Wang, Z. (2017). Protective role of α-lipoic acid in hyperuricemia-induced endothelial dysfunction. Experimental and Therapeutic Medicine, 13, 3047-3054. https://doi.org/10.3892/etm.2017.4345
MLA
Zou, H., Wang, H., Liu, T., Li, X., Zhu, X., Wang, Z."Protective role of α-lipoic acid in hyperuricemia-induced endothelial dysfunction". Experimental and Therapeutic Medicine 13.6 (2017): 3047-3054.
Chicago
Zou, H., Wang, H., Liu, T., Li, X., Zhu, X., Wang, Z."Protective role of α-lipoic acid in hyperuricemia-induced endothelial dysfunction". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3047-3054. https://doi.org/10.3892/etm.2017.4345