Open Access

Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke

  • Authors:
    • Yi Chen
    • Huasheng Tong
    • Zhiguo Pan
    • Dongxin Jiang
    • Xinqin Zhang
    • Junming Qiu
    • Lei Su
    • Minzhou Zhang
  • View Affiliations

  • Published online on: May 8, 2017     https://doi.org/10.3892/etm.2017.4444
  • Pages: 3408-3416
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the protective effect of Xuebijing injection (XBJ) on lung injury in heat‑stroke rats and the underlying mechanisms. In total, 54 rats were randomly assigned to non‑thermal, saline vehicle and XBJ groups. The rectal temperature (Tc), mean arterial pressure (MAP) and respiratory rate (RR) of the rats were recorded. The time‑point of heat stroke and the time of survival were assessed, and indicators of arterial blood gas were regularly measured from 0 to 60 min. The concentration of tumor necrosis factor‑α (TNF‑α), interleukin‑1β (IL‑1β) and IL‑10 was also determined. At the end of the experiment, lung tissue was harvested for histopathological analysis. Inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) expression was measured by immunohistochemistry. Terminal deoxynucleotidyl transferase‑mediated dUTP nick‑end labeling was used to measure apoptosis. XBJ pretreatment prolonged the decline of clinical characteristics, as demonstrated by increases in Tc, MAP, RR and indicators in arterial blood gas in rats under heat stress. The time until heat stroke and the survival time in the Saline group were shorter than in rats treated with XBJ. The expression of iNOS in lung tissue and the concentration of TNF‑α, IL‑1β and IL‑10 in the bronchoalveolar lavage fluid of rats treated with saline was higher than in rats with XBJ pre‑treatment. Contrarily, SOD expression in rats treated with saline was decreased compared with that in rats treated with XBJ. Moreover, the apoptotic rate in the lung tissues of rats with saline treatment was higher than that in rats treated with XBJ. In conclusion, XBJ delayed the development of heat stroke and increased the survival time in rats under heat‑stress by ameliorating pulmonary failure and acute lung injury. The underlying mechanisms of this effect may be the reduction of inflammatory cytokines as well as attenuation of oxidative stress and apoptosis by XBJ.

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June-2017
Volume 13 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen Y, Tong H, Pan Z, Jiang D, Zhang X, Qiu J, Su L and Zhang M: Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke. Exp Ther Med 13: 3408-3416, 2017
APA
Chen, Y., Tong, H., Pan, Z., Jiang, D., Zhang, X., Qiu, J. ... Zhang, M. (2017). Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke. Experimental and Therapeutic Medicine, 13, 3408-3416. https://doi.org/10.3892/etm.2017.4444
MLA
Chen, Y., Tong, H., Pan, Z., Jiang, D., Zhang, X., Qiu, J., Su, L., Zhang, M."Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke". Experimental and Therapeutic Medicine 13.6 (2017): 3408-3416.
Chicago
Chen, Y., Tong, H., Pan, Z., Jiang, D., Zhang, X., Qiu, J., Su, L., Zhang, M."Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke". Experimental and Therapeutic Medicine 13, no. 6 (2017): 3408-3416. https://doi.org/10.3892/etm.2017.4444