Open Access

Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer

  • Authors:
    • Fei Wu
    • Jun Chen
    • Li‑Mei Fan
    • Kai Liu
    • Ning Zhang
    • Shi‑Wen Li
    • He Zhu
    • Hai‑Cheng Gao
  • View Affiliations

  • Published online on: May 22, 2017     https://doi.org/10.3892/etm.2017.4494
  • Pages: 127-130
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of rutin treatment on the expression of glycogen synthase kinase (GSK)-3β and tumor necrosis factor (TNF)‑α in A549 human lung carcinoma cells. The A549 cells were divided into control, cisplatin and rutin (low, middle and high) groups. ELISA and western blot analysis of TNF‑α expression, 4',6-diamino-2‑phenylindole (DAPI) staining and GSK‑3β immunofluorescence staining were used to investigate the effect of rutin in the human lung carcinoma cells, using cisplatin as a positive control. TNF‑α expression was significantly higher in the rutin and cisplatin groups compared with the control group. Additionally, DAPI staining revealed that the number of apoptotic cells was higher in the rutin and cisplatin groups compared with the control group, and immunofluorescence showed that the expression of GSK‑3β in the cisplatin and rutin groups was significantly higher compared with that in the control group. The results of the present study suggest that rutin promotes the TNF‑α‑induced apoptosis of A549 human lung carcinoma cells. Furthermore, rutin may be able to regulate the expression of GSK‑3β protein in these cells.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu F, Chen J, Fan LM, Liu K, Zhang N, Li SW, Zhu H and Gao HC: Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer. Exp Ther Med 14: 127-130, 2017
APA
Wu, F., Chen, J., Fan, L., Liu, K., Zhang, N., Li, S. ... Gao, H. (2017). Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer. Experimental and Therapeutic Medicine, 14, 127-130. https://doi.org/10.3892/etm.2017.4494
MLA
Wu, F., Chen, J., Fan, L., Liu, K., Zhang, N., Li, S., Zhu, H., Gao, H."Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer". Experimental and Therapeutic Medicine 14.1 (2017): 127-130.
Chicago
Wu, F., Chen, J., Fan, L., Liu, K., Zhang, N., Li, S., Zhu, H., Gao, H."Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer". Experimental and Therapeutic Medicine 14, no. 1 (2017): 127-130. https://doi.org/10.3892/etm.2017.4494