Reducing capacity and enzyme activity of chromate reductase in a ChrT‑engineered strain

  • Authors:
    • Simin Zhou
    • Lanlan Dong
    • Peng Deng
    • Yan Jia
    • Qunhua Bai
    • Jieying Gao
    • Hong Xiao
  • View Affiliations

  • Published online on: July 11, 2017     https://doi.org/10.3892/etm.2017.4775
  • Pages: 2361-2366
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Abstract

In order to remediate the metal‑contaminated soil and water ecosystems with microorganisms, an engineered strain, which contained the chromate reductase ChrT gene from Serratia sp. S2, was studied in detail for its Cr (VI) reduction efficiency, optimal culture condition and chromate reductase activity. Results demonstrated that the engineered strain had a high Cr (VI) reduction rate of up to 40% at a concentration of 50 mg/l after being cultured for 48 h. Additionally, the optimal culture conditions were pH 7.0 and 37˚C. Furthermore, the carbon sources and metal cations exhibited significant effects on the Cr (VI) reduction rate of the engineered bacterium. Sodium lactate, sodium acetate, Cu2+, Co2+ and Pb2+ were positively correlated with the reduction rate. Chromate reductase was soluble and presented in the cytoplasm. Furthermore, the enzymatic activity with nicotinamide adenine dinucleotide phosphate, which was as an electron donor, reached 14.83 U/mg.

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September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhou S, Dong L, Deng P, Jia Y, Bai Q, Gao J and Xiao H: Reducing capacity and enzyme activity of chromate reductase in a ChrT‑engineered strain. Exp Ther Med 14: 2361-2366, 2017
APA
Zhou, S., Dong, L., Deng, P., Jia, Y., Bai, Q., Gao, J., & Xiao, H. (2017). Reducing capacity and enzyme activity of chromate reductase in a ChrT‑engineered strain. Experimental and Therapeutic Medicine, 14, 2361-2366. https://doi.org/10.3892/etm.2017.4775
MLA
Zhou, S., Dong, L., Deng, P., Jia, Y., Bai, Q., Gao, J., Xiao, H."Reducing capacity and enzyme activity of chromate reductase in a ChrT‑engineered strain". Experimental and Therapeutic Medicine 14.3 (2017): 2361-2366.
Chicago
Zhou, S., Dong, L., Deng, P., Jia, Y., Bai, Q., Gao, J., Xiao, H."Reducing capacity and enzyme activity of chromate reductase in a ChrT‑engineered strain". Experimental and Therapeutic Medicine 14, no. 3 (2017): 2361-2366. https://doi.org/10.3892/etm.2017.4775