Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics

  • Authors:
    • Jianting Yang
    • Qian Li
    • Liujiao Bian
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/etm.2017.4853
  • Pages: 3288-3298
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Abstract

The interaction between TEM-1 β-lactamase and antibiotics is very important in the hydrolysis of antibiotics. In the present study, the recognition and binding of TEM‑1 β‑lactamase with three β‑lactam antibiotics, including penicillin G, cefalexin and cefoxitin, was investigated by fluorescence and ultraviolet‑visible absorption spectra in combination with molecular docking in the temperature range of 278‑288 K and under simulated physiological conditions. The results demonstrated that the fluorescence emissions of TEM‑1 β‑lactamase were extinguished by static quenching and the energy of TEM‑1 β‑lactamase was transferred in a non‑radioactive manner. The binding of TEM‑1 β‑lactamase with the three antibiotics was a spontaneously exothermic process, with binding constants of 1.41x107, 7.81x106 and 5.43x104 at 278 K. Furthermore, binding was driven by enthalpy change and the binding forces between them were mainly hydrogen bonding and Van der Waals forces. A TEM‑1 β‑lactamase only bound with one antibiotic at a time and the binding capacity between them was closely associated with the functional groups and flexibility in the antibiotics. In addition, a conformational change occurred in the TEM‑1 β‑lactamases when they bound with the three antibiotics and TEM‑1 β‑lactamase‑antibiotic complexes were formed. The present study provided an insight into the recognition and binding of TEM‑1 β‑lactamase with β‑lactam antibiotics, which may be helpful for designing a novel substrate for TEM‑1 β‑lactamase and developing novel antibiotics that are resistant to the enzyme.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yang J, Li Q and Bian L: Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics. Exp Ther Med 14: 3288-3298, 2017
APA
Yang, J., Li, Q., & Bian, L. (2017). Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics. Experimental and Therapeutic Medicine, 14, 3288-3298. https://doi.org/10.3892/etm.2017.4853
MLA
Yang, J., Li, Q., Bian, L."Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics". Experimental and Therapeutic Medicine 14.4 (2017): 3288-3298.
Chicago
Yang, J., Li, Q., Bian, L."Spectroscopic analysis and docking simulation on the recognition and binding of TEM-1 β-lactamase with β-lactam antibiotics". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3288-3298. https://doi.org/10.3892/etm.2017.4853