Open Access

Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment

  • Authors:
    • Yinghui Deng
    • Na Lin
    • Leiyun Wu
    • Qaing Jia
    • Hua Liu
  • View Affiliations

  • Published online on: August 16, 2017     https://doi.org/10.3892/etm.2017.4950
  • Pages: 3632-3636
  • Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to determine whether the expression of lectin‑like oxidized LDL receptor 1 (LOX‑1) could be induced by oxidized low‑density lipoprotein (Ox‑LDL) and interleukin 1β (IL‑1β) in human mesangial cells (HMCs). Oil Red O staining was used to observe the uptake of Ox‑LDL by HMCs stimulated with IL‑1β, and reverse transcription‑quantitative polymerase chain reaction analysis and western blotting were used to examine the expression of LOX‑1 in HMC following Ox‑LDL and IL‑1β treatment. Uptake of Ox‑LDL by HMCs was upregulated upon stimulation with IL‑1β. Furthermore, Ox‑LDL (10‑40 µg/ml) treatment induced LOX‑1 mRNA and protein expression in a dose‑dependent manner. In addition, when HMCs were treated with IL‑1β and Ox‑LDL, the expression of LOX‑1 was enhanced further. These results indicated that inhibiting LOX‑1 expression or inhibiting the Ox‑LDL/LOX‑1 signaling axis may be a potential novel method for treating renal disease.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Deng Y, Lin N, Wu L, Jia Q and Liu H: Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment. Exp Ther Med 14: 3632-3636, 2017
APA
Deng, Y., Lin, N., Wu, L., Jia, Q., & Liu, H. (2017). Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment. Experimental and Therapeutic Medicine, 14, 3632-3636. https://doi.org/10.3892/etm.2017.4950
MLA
Deng, Y., Lin, N., Wu, L., Jia, Q., Liu, H."Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment". Experimental and Therapeutic Medicine 14.4 (2017): 3632-3636.
Chicago
Deng, Y., Lin, N., Wu, L., Jia, Q., Liu, H."Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3632-3636. https://doi.org/10.3892/etm.2017.4950