Antimicrobial resistance and prevalence of CvfB, SEK and SEQ genes among Staphylococcus aureus isolates from paediatric patients with bloodstream infections

  • Authors:
    • Bing‑Shao Liang
    • Yan‑Mei Huang
    • Yin‑Shuang Chen
    • Hui Dong
    • Jia‑Liang Mai
    • Yong‑Qiang Xie
    • Hua‑Min Zhong
    • Qiu‑Lian Deng
    • Yan Long
    • Yi‑Yu Yang
    • Si‑Tang Gong
    • Zhen‑Wen Zhou
  • View Affiliations

  • Published online on: September 22, 2017     https://doi.org/10.3892/etm.2017.5199
  • Pages: 5143-5148
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Abstract

Staphylococcus aureus (S. aureus) is one of the most frequently isolated pathogens in neonatal cases of early and late‑onset sepsis. Drug resistance profiles and carriage of toxin genes may affect the treatment and outcome of an infection. The present study aimed to determine the antimicrobial resistance patterns and frequencies of the toxin‑associated genes conserved virulence factor B (CvfB), staphylococcal enterotoxin Q (SEQ) and staphylococcal enterotoxin K (SEK) among S. aureus isolates recovered from paediatric patients with bloodstream infections (BSIs) in Guangzhou (China). Of the 53 isolates, 43.4% were methicillin‑resistant S. aureus (MRSA), and resistance rates to penicillin, erythromycin, clindamycin, trimethoprim/sulfamethoxazole, tetracycline, and ciprofloxacin of 92.5, 66.0, 62.3, 13.2, 20.8 and 1.9% were recorded, respectively. However, no resistance to nitrofurantoin, dalfopristin/quinupristin, rifampicin, gentamicin, linezolid or vancomycin was detected. Resistance to erythromycin, clindamycin and tetracycline in the MRSA group was significantly higher than that in the methicillin‑susceptible S. aureus (MSSA) group. No significant differences in antimicrobial resistance patterns were noted between two age groups (≤1 year and >1 year). The proportion of S. aureus isolates positive for CvfB, SEQ and SEK was 100, 34.0 and 35.8%, respectively, with 24.5% (13/53) of strains carrying all three genes. Compared with those in MSSA isolates, the rates of SEK, SEQ and SEK + SEQ carriage among MRSA isolates were significantly higher. Correlations were identified between the carriage of SEQ, SEK and SEQ + SEK genes and MRSA (contingency coefficient 0.500, 0.416, 0.546, respectively; P<0.01). In conclusion, MRSA isolated from the blood of paediatric patients with BSIs not only exhibited higher rates of antimicrobial resistance than MSSA from the same source, but also more frequently harboured SEK and SEQ genes. The combination of the two aspects influenced the dissemination of MRSA among children. The present study clarified the characteristics of BSI‑associated S. aureus and enhanced the current understanding of the pathogenicity and treatment of MRSA.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liang BS, Huang YM, Chen YS, Dong H, Mai JL, Xie YQ, Zhong HM, Deng QL, Long Y, Yang YY, Yang YY, et al: Antimicrobial resistance and prevalence of CvfB, SEK and SEQ genes among Staphylococcus aureus isolates from paediatric patients with bloodstream infections. Exp Ther Med 14: 5143-5148, 2017
APA
Liang, B., Huang, Y., Chen, Y., Dong, H., Mai, J., Xie, Y. ... Zhou, Z. (2017). Antimicrobial resistance and prevalence of CvfB, SEK and SEQ genes among Staphylococcus aureus isolates from paediatric patients with bloodstream infections. Experimental and Therapeutic Medicine, 14, 5143-5148. https://doi.org/10.3892/etm.2017.5199
MLA
Liang, B., Huang, Y., Chen, Y., Dong, H., Mai, J., Xie, Y., Zhong, H., Deng, Q., Long, Y., Yang, Y., Gong, S., Zhou, Z."Antimicrobial resistance and prevalence of CvfB, SEK and SEQ genes among Staphylococcus aureus isolates from paediatric patients with bloodstream infections". Experimental and Therapeutic Medicine 14.5 (2017): 5143-5148.
Chicago
Liang, B., Huang, Y., Chen, Y., Dong, H., Mai, J., Xie, Y., Zhong, H., Deng, Q., Long, Y., Yang, Y., Gong, S., Zhou, Z."Antimicrobial resistance and prevalence of CvfB, SEK and SEQ genes among Staphylococcus aureus isolates from paediatric patients with bloodstream infections". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5143-5148. https://doi.org/10.3892/etm.2017.5199