Open Access

Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice

  • Authors:
    • Xueqin Song
    • Ya Zhang
    • Zhigang Hou
    • Hongran Wu
    • Shan Lu
    • Jin Tang
    • Xuexiao Chen
    • Hongying Cui
    • Yuan Li
    • Yue Bi
    • Weisong Duan
    • Zhongyao Li
    • Chunyan Li
  • View Affiliations

  • Published online on: December 11, 2017     https://doi.org/10.3892/etm.2017.5610
  • Pages:1825-1830
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disease caused by the absence of dystrophin. Vascular endothelial growth factor (VEGF) is a heparin‑binding dimeric glycoprotein and principal angiogenic factor stimulating the migration, proliferation and expression of various genes in endothelial cells. Recently, VEGF was demonstrated to exhibit an antiapoptotic and direct myogenic effect, as well as to enhance muscle force restoration subsequent to traumatic injury. Therefore, the present study attempted to assess the muscle damage of VEGF overexpression in mdx mice. Adeno‑associated virus serotype 9 (AAV9)‑VEGF was administered intravenously to mdx mice. At 4 weeks after injection, VEGF was observed to be upregulated in the tibialis anterior muscle. In addition, the serum creatine kinase levels were significantly reduced and fatigue was slowed down, whereas the limb grip strength and weight of mice were markedly increased compared with the saline‑treated mdx mice. Furthermore, significantly reduced inflammation and necrosis areas were observed in the muscle tissues of mice in the AAV9‑VEGF group. These results suggested that AAV9‑mediated VEGF gene overexpression was able to improve the muscle damage in mdx mice.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Song, X., Zhang, Y., Hou, Z., Wu, H., Lu, S., Tang, J. ... Li, C. (2018). Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice. Experimental and Therapeutic Medicine, 15, 1825-1830. https://doi.org/10.3892/etm.2017.5610
MLA
Song, X., Zhang, Y., Hou, Z., Wu, H., Lu, S., Tang, J., Chen, X., Cui, H., Li, Y., Bi, Y., Duan, W., Li, Z., Li, C."Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice". Experimental and Therapeutic Medicine 15.2 (2018): 1825-1830.
Chicago
Song, X., Zhang, Y., Hou, Z., Wu, H., Lu, S., Tang, J., Chen, X., Cui, H., Li, Y., Bi, Y., Duan, W., Li, Z., Li, C."Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1825-1830. https://doi.org/10.3892/etm.2017.5610