Open Access

Human adipose‑derived mesenchymal stem cell‑conditioned media suppresses inflammatory bone loss in a lipopolysaccharide‑induced murine model

  • Authors:
    • Yu Li
    • Xin Gao
    • Jinbing Wang
  • View Affiliations

  • Published online on: December 7, 2017     https://doi.org/10.3892/etm.2017.5606
  • Pages:1839-1846
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Conditioned media (CM) from mesenchymal stem cells (MSCs) contains various cytokines, growth factors and microRNAs, which may serve important roles in modulating the inflammatory process. However, the effect of MSC‑CM on inflammatory bone loss remains unknown. The present study investigated the effects of conditioned media from human adipose‑derived mesenchymal stem cells (AMSC‑CM) on the prevention of lipopolysaccharide (LPS)‑mediated bone loss in mice. To investigate the underlying mechanisms of this effect, the effects of AMSC‑CM on serum levels of inflammation‑associated cytokines [tumor necrosis factor (TNF)‑α, interleukin (IL)‑1, IL‑6 and IL‑10] in LPS‑treated mice, in addition to their mRNA expression in LPS‑treated macrophages, was investigated. Micro‑computed tomography and histological analysis revealed that AMSC‑CM administration effectively inhibited LPS‑induced bone destruction in vivo. ELISA analysis indicated that AMSC‑CM significantly reduced the serum levels of proinflammatory cytokines (TNF‑α, IL‑1 and IL‑6) in LPS‑treated mice. Furthermore, AMSC‑CM treatment significantly decreased the mRNA expression levels of TNF‑α, IL‑1 and IL‑6 in macrophages treated with LPS. These findings indicate that AMSC‑CM inhibits LPS‑induced bone loss by decreasing the production of proinflammatory cytokines, suggesting that the use of AMSC‑CM may be a potential therapeutic strategy for the treatment of inflammatory bone loss.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Li, Y., Gao, X., & Wang, J. (2018). Human adipose‑derived mesenchymal stem cell‑conditioned media suppresses inflammatory bone loss in a lipopolysaccharide‑induced murine model. Experimental and Therapeutic Medicine, 15, 1839-1846. https://doi.org/10.3892/etm.2017.5606
MLA
Li, Y., Gao, X., Wang, J."Human adipose‑derived mesenchymal stem cell‑conditioned media suppresses inflammatory bone loss in a lipopolysaccharide‑induced murine model". Experimental and Therapeutic Medicine 15.2 (2018): 1839-1846.
Chicago
Li, Y., Gao, X., Wang, J."Human adipose‑derived mesenchymal stem cell‑conditioned media suppresses inflammatory bone loss in a lipopolysaccharide‑induced murine model". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1839-1846. https://doi.org/10.3892/etm.2017.5606