Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses

  • Authors:
    • Yoko Motokawa
    • Michifumi Kokubo
    • Nobuo Kuwabara
    • Ken‑Ichiro Tatematsu
    • Hideki Sezutsu
    • Hideyuki Takahashi
    • Koichi Sakakura
    • Kazuaki Chikamatsu
    • Shigeki Takeda
  • View Affiliations

  • Published online on: January 4, 2018     https://doi.org/10.3892/etm.2018.5703
  • Pages: 2512-2518
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recent clinical trials with the aim of developing tumor antigen (TA)‑specific cancer vaccines against a number of malignancies have focused on the identification of TAs presented by tumor cells and recognized by T cells. In the present study, the TA melanoma antigen family A4 (MAGE‑A4) protein was produced using a transgenic (TG) silkworm system. Using in vitro stimulation, it was subsequently determined whether MAGE‑A4 protein induced MAGE‑A4‑specific T cells from peripheral blood mononuclear cells of healthy donors. TG silkworm lines expressing a MAGE‑A4 gene under an upstream activating sequence (UAS) were mated with those expressing a yeast transcription activator protein (GAL4) at the middle silk glands (MSGs) and embryos that harbored both the GAL4 and UAS constructs were selected. Recombinant MAGE‑A4 protein was extracted from the MSGs of TG silkworms and evaluated using SDS‑PAGE and western blot analysis. It was observed that MAGE‑A4 produced by the TG silkworm system successfully induced MAGE‑A4‑specific CD4+ T cell responses. Furthermore, MAGE‑A4‑specific CD4+ T cells recognized antigen‑presenting cells when pulsed with a MAGE‑A4+ tumor cell lysate. The present data suggests that recombinant tumor antigen production using the TG silkworm system may be a novel tool in the preparation of cancer vaccines.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Motokawa Y, Kokubo M, Kuwabara N, Tatematsu KI, Sezutsu H, Takahashi H, Sakakura K, Chikamatsu K and Takeda S: Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses. Exp Ther Med 15: 2512-2518, 2018
APA
Motokawa, Y., Kokubo, M., Kuwabara, N., Tatematsu, K., Sezutsu, H., Takahashi, H. ... Takeda, S. (2018). Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses. Experimental and Therapeutic Medicine, 15, 2512-2518. https://doi.org/10.3892/etm.2018.5703
MLA
Motokawa, Y., Kokubo, M., Kuwabara, N., Tatematsu, K., Sezutsu, H., Takahashi, H., Sakakura, K., Chikamatsu, K., Takeda, S."Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses". Experimental and Therapeutic Medicine 15.3 (2018): 2512-2518.
Chicago
Motokawa, Y., Kokubo, M., Kuwabara, N., Tatematsu, K., Sezutsu, H., Takahashi, H., Sakakura, K., Chikamatsu, K., Takeda, S."Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2512-2518. https://doi.org/10.3892/etm.2018.5703