BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures

  • Authors:
    • Xu Yan
    • Zhenhua Zhou
    • Lixin Guo
    • Zhaochi Zeng
    • Zhongkai Guo
    • Qingdong Shao
    • Weidong Xu
  • View Affiliations

  • Published online on: June 21, 2018     https://doi.org/10.3892/etm.2018.6339
  • Pages: 1381-1388
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Abstract

Bone fractures are a worldwide public health concern. Previous studies have demonstrated that bone morphogenetic protein‑7 (BMP7) gene transfer or mesenchymal stem cells (MSCs) transplantation may be a promising novel therapeutic approach. Therefore, the aim of the present study was to observe the effect of bone BMP7 transfer to MSCs on fracture healing. Bone marrow‑derived MSCs (BMSCs) from New Zealand white rabbits were isolated and identified using flow cytometry. A recombinant BMP7 overexpressing adenovirus vector (Adv) was constructed and transfected into BMSCs. The expression of BMP7 was detected by reverse transcription‑polymerase chain reaction, immunofluorescence and western blotting. The present study additionally investigated the effect of BMP7 on the differentiation capacity of BMSCs. Finally, tissue‑engineered bone was created with support material to verify the effect of BMP7‑BMSCs on fracture healing. The results demonstrated that the expression of BMP7 was increased at the mRNA and protein levels in BMSCs following transfection with BMP7 overexpressing Adv. The results additionally demonstrated that the expression of BMP7 enhanced the differentiation capacity of bone marrow mesenchymal stem cells and had a promotional effect on fracture healing. Overall, these data suggest that Adv‑BMP7 is useful for introducing foreign genes into BMSCs and will be a powerful gene therapy tool for bone regeneration and other tissue engineering applications in the future.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yan X, Zhou Z, Guo L, Zeng Z, Guo Z, Shao Q and Xu W: BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures. Exp Ther Med 16: 1381-1388, 2018
APA
Yan, X., Zhou, Z., Guo, L., Zeng, Z., Guo, Z., Shao, Q., & Xu, W. (2018). BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures. Experimental and Therapeutic Medicine, 16, 1381-1388. https://doi.org/10.3892/etm.2018.6339
MLA
Yan, X., Zhou, Z., Guo, L., Zeng, Z., Guo, Z., Shao, Q., Xu, W."BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures". Experimental and Therapeutic Medicine 16.2 (2018): 1381-1388.
Chicago
Yan, X., Zhou, Z., Guo, L., Zeng, Z., Guo, Z., Shao, Q., Xu, W."BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1381-1388. https://doi.org/10.3892/etm.2018.6339