Open Access

Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair

  • Authors:
    • Zheng Chen
    • Qianqian Chen
    • Haitao Du
    • Lijuan Xu
    • Jun Wan
  • View Affiliations

  • Published online on: May 29, 2018     https://doi.org/10.3892/etm.2018.6233
  • Pages: 821-829
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Abstract

The present study intended to observe the homing capability and therapeutic effect of CXC chemokine receptor 4 (CXCR‑4) gene overexpressed bone marrow mesenchymal stem cells (BMSCs) on colitis, and to study the possible mechanisms involved. BMSCs were derived from male BALB/c mice and CXCR‑4 gene was transfected into BMSCs by the utilization of the lentiviral vector. The expression of CXCR‑4 gene was analyzed and the biological characteristics, and vitality of BMSCs and CXCR‑4 gene overexpressed BMSCs (CXCR‑BMSCs) were detected. The chemotaxis assay was performed to investigate migration in vitro. Colitis was induced by TNBS in female BALB/c mice. BMSCs and CXCR‑BMSCs were injected into experimental models intravenously. The homing of cells was confirmed by fluorescence observation and Sry gene detection. Clinical manifestation and histological changes were also evaluated. The expression levels of occludin and vascular endothelial growth factor (VEGF) were detected to measure mucosal repair. Furthermore, CXCR‑4 gene was successfully transfected into BMSCs by the utilization of lentiviral vector. Results indicated that overexpression of CXCR‑4 gene did not influence the biological characteristics and vitality of BMSCs, but enhanced the capability of migration and homing of BMSCs in vitro and in vivo. Notably, CXCR‑BMSCs had an improved effect on treating colitis, and the expression levels of Occludin and VEGF were higher. The results suggested that overexpression of CXCR‑4 gene may enhance BMSC homing to damaged intestinal mucosa and their curative effect on colitis. The present findings indicated that using lentiviral vector to transfect CXCR‑4 gene into BMSCs may be a potential method to improve the outcomes of BMSCs treatment on inflammatory bowel disease.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen Z, Chen Q, Du H, Xu L and Wan J: Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. Exp Ther Med 16: 821-829, 2018
APA
Chen, Z., Chen, Q., Du, H., Xu, L., & Wan, J. (2018). Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. Experimental and Therapeutic Medicine, 16, 821-829. https://doi.org/10.3892/etm.2018.6233
MLA
Chen, Z., Chen, Q., Du, H., Xu, L., Wan, J."Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair". Experimental and Therapeutic Medicine 16.2 (2018): 821-829.
Chicago
Chen, Z., Chen, Q., Du, H., Xu, L., Wan, J."Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair". Experimental and Therapeutic Medicine 16, no. 2 (2018): 821-829. https://doi.org/10.3892/etm.2018.6233