Metformin exerts anti‑inflammatory effects on mouse colon smooth muscle cells in vitro

  • Authors:
    • Ahmed Al‑Dwairi
    • Mohammad Alqudah
    • Othman Al‑Shboul
    • Mahmoud Alfaqih
    • Dana Alomari
  • View Affiliations

  • Published online on: May 25, 2018     https://doi.org/10.3892/etm.2018.6222
  • Pages: 985-992
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Inflammatory bowel disease (IBD) is a chronic incurable condition characterized by relapsing inflammation of the gut. Intestinal smooth muscle cells (SMCs) are affected structurally and functionally during IBD due to excessive production of different inflammatory mediators. Metformin is a widely used antidiabetic agent known to exert several anti‑inflammatory effects in different tissues independently from its hypoglycemic effect. The aim of the present study was to investigate the effect of metformin on expression and secretion of different cytokines and chemokines from mouse colon SMCs (CSMCs) following induction of inflammation with lipopolysaccharide (LPS) in vitro. CSMCs from male BALB/c mice were isolated and cultured in Dulbecco's modified Eagle's medium and treated with LPS (1 µg/ml) and 0, 5, 10 or 20 mM metformin for 24 h. Expression and secretion of tumor necrosis factor‑α (TNF‑α), interleukin‑1α (IL‑1α), macrophage colony stimulating factor (M‑CSF), T cell activation gene‑3 (TCA‑3) and stromal cell‑derived factor‑1 (SDF‑1) was evaluated by ELISA. LPS‑treated CSMCs demonstrated significantly increased expression of TNF‑α, IL‑1α, M‑CSF, TCA‑3 and SDF‑1 when compared with the control group (P<0.05). Co‑treatment with metformin (5 and 10 mM) significantly reduced their expression by ~20‑40% when compared with LPS treatment alone (P<0.05). Furthermore, secretion of TNF‑α, IL‑1α, M‑CSF and TCA‑3 into the conditioned media was significantly decreased by metformin (5 and 10 mM; P<0.05). In addition, metformin decreased levels of LPS‑induced nuclear factor‑κB phosphorylation. These data suggest that metformin may provide beneficial anti‑inflammatory effects on CSMCs and it may be utilized as an adjunct therapy for patients suffering from IBD.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Al‑Dwairi A, Alqudah M, Al‑Shboul O, Alfaqih M and Alomari D: Metformin exerts anti‑inflammatory effects on mouse colon smooth muscle cells in vitro. Exp Ther Med 16: 985-992, 2018
APA
Al‑Dwairi, A., Alqudah, M., Al‑Shboul, O., Alfaqih, M., & Alomari, D. (2018). Metformin exerts anti‑inflammatory effects on mouse colon smooth muscle cells in vitro. Experimental and Therapeutic Medicine, 16, 985-992. https://doi.org/10.3892/etm.2018.6222
MLA
Al‑Dwairi, A., Alqudah, M., Al‑Shboul, O., Alfaqih, M., Alomari, D."Metformin exerts anti‑inflammatory effects on mouse colon smooth muscle cells in vitro". Experimental and Therapeutic Medicine 16.2 (2018): 985-992.
Chicago
Al‑Dwairi, A., Alqudah, M., Al‑Shboul, O., Alfaqih, M., Alomari, D."Metformin exerts anti‑inflammatory effects on mouse colon smooth muscle cells in vitro". Experimental and Therapeutic Medicine 16, no. 2 (2018): 985-992. https://doi.org/10.3892/etm.2018.6222