Open Access

Effects of transplanted adipose derived stem cells on the expressions of α‑SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears

  • Authors:
    • Haihan Chu
    • Yunpeng Wang
    • Xiuchun Wang
    • Xianhui Song
    • Huaqing Liu
    • Xue Li
  • View Affiliations

  • Published online on: June 29, 2018     https://doi.org/10.3892/etm.2018.6383
  • Pages: 1729-1734
  • Copyright: © Chu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To study the effects of transplanted adipose derived stem cells (ADSCs) on the expressions of α-smooth muscle actin (α‑SMA) and decorin (DCN) in fibroblasts of hypertrophic scar tissues in rabbit ears. Twelve New Zealand white rabbits were selected; the normal subcutaneous adipose tissues in inguinal region were removed, ADSCs were extracted via enzyme digestion, cultured in Dulbecco's modified Eagle's medium (DMEM) and inoculated into the culture dish (3-5x104 cells/ml). After the rabbit ear hypertrophic scar model was established successfully, the fibroblasts of hypertrophic scar tissues in rabbit ears were separated and cultured using the mechanical method combined with enzyme digestion, and the ADSCs and scar fibroblasts were cultured in non-contact Transwell co-culture system for 21 days (experimental group); the corresponding scar fibroblasts were cultured in an ordinary 6-well plate without any treatment for 21 days (control group). The content of collagen Ι in fibroblasts was detected using the enzyme-linked immunosorbent assay (ELISA) kit, the mRNA expressions of α‑SMA and DCN were detected via reverse transcription-polymerase chain reaction (RT-PCR), the protein expressions of α‑SMA and DCN were detected via western blot analysis, and the expressions and distribution of α‑SMA and DCN were detected via immunofluorescence assay. The results of ELISA showed that the content of collagen I in experimental group was decreased significantly (p<0.01). The results of RT-PCR and western blot analysis revealed that the mRNA and protein expression levels of α‑SMA were significantly decreased (P<0.01, but those of DCN were significantly increased (p<0.01). Moreover, the results of immunofluorescence assay showed that the expression of α‑SMA in experimental group was significantly decreased, while the expression of DCN was significantly increased. ADSCs can inhibit the mRNA and protein expressions of α‑SMA and promote the mRNA and protein expressions of DCN in in vitro culture system, and they are expected to be used in the prevention and treatment of pathological scars.
View Figures
View References

Related Articles

Journal Cover

September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chu H, Wang Y, Wang X, Song X, Liu H and Li X: Effects of transplanted adipose derived stem cells on the expressions of α‑SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears. Exp Ther Med 16: 1729-1734, 2018
APA
Chu, H., Wang, Y., Wang, X., Song, X., Liu, H., & Li, X. (2018). Effects of transplanted adipose derived stem cells on the expressions of α‑SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears. Experimental and Therapeutic Medicine, 16, 1729-1734. https://doi.org/10.3892/etm.2018.6383
MLA
Chu, H., Wang, Y., Wang, X., Song, X., Liu, H., Li, X."Effects of transplanted adipose derived stem cells on the expressions of α‑SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears". Experimental and Therapeutic Medicine 16.3 (2018): 1729-1734.
Chicago
Chu, H., Wang, Y., Wang, X., Song, X., Liu, H., Li, X."Effects of transplanted adipose derived stem cells on the expressions of α‑SMA and DCN in fibroblasts of hypertrophic scar tissues in rabbit ears". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1729-1734. https://doi.org/10.3892/etm.2018.6383