miR‑365b‑3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis

  • Authors:
    • Yunfei Qu
    • Ning Zhang
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  • Published online on: September 11, 2018     https://doi.org/10.3892/etm.2018.6720
  • Pages: 4239-4245
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Abstract

Abnormal proliferation and migration of vascular smooth muscle cells serves a crucial role in the development of atherosclerosis. Previous studies have suggested that some microRNAs (miRs) are involved in this process; however, the associated underlying molecular mechanism is unclear. In present study, human coronary artery smooth muscle cells (HCASMCs) were used to explore the function of miR‑365b‑3p in the coronary atherosclerosis. It was indicated that platelet‑derived growth factor‑BB (PDGF‑BB) treatment inhibited miR‑365b‑3p expression and upregulated the expression of a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) in HCASMCs. Subsequently, miR‑365b‑3p mimic was transfected in HCASMCs to explore the function of this miR. The results of reverse transcription‑quantitative polymerase chain reaction and western blot analysis indicated that overexpression of miR‑365b‑3p significantly downregulated ADAMTS1 expression. Functional assay results revealed that overexpression of miR‑365b‑3p significantly attenuated PDGF‑BB‑induced proliferation and migration of HCASMCs. Furthermore, the dual‑luciferase reporter assay results confirmed that ADAMTS1 is a direct target gene of miR‑365b‑3p. This discovery proposed a novel channel of communication between ADAMTS1 and HCASMCs, and suggests a potential therapeutic approach for coronary atherosclerosis.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Qu Y and Qu Y: miR‑365b‑3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis. Exp Ther Med 16: 4239-4245, 2018
APA
Qu, Y., & Qu, Y. (2018). miR‑365b‑3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis. Experimental and Therapeutic Medicine, 16, 4239-4245. https://doi.org/10.3892/etm.2018.6720
MLA
Qu, Y., Zhang, N."miR‑365b‑3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis". Experimental and Therapeutic Medicine 16.5 (2018): 4239-4245.
Chicago
Qu, Y., Zhang, N."miR‑365b‑3p inhibits the cell proliferation and migration of human coronary artery smooth muscle cells by directly targeting ADAMTS1 in coronary atherosclerosis". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4239-4245. https://doi.org/10.3892/etm.2018.6720