GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG

  • Authors:
    • Yibin Zhou
    • Huiyu Chang
    • Beibei Yang
  • View Affiliations

  • Published online on: October 3, 2018     https://doi.org/10.3892/etm.2018.6826
  • Pages: 5318-5326
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Abstract

GATA4, a member of the GATA family, serves a key function in several types of cancer, including hepatoblastoma, gastric cancer and breast cancer. However, the function of GATA4 in nasopharyngeal cancer (NPC) is largely unknown. The present study revealed that GATA4 was upregulated in NPC tissue samples and the NPC cell line, 5‑8F. Furthermore, the expression of GATA4 was associated with tumor size, metastasis and poor prognosis. Transwell invasion and wound healing analyses demonstrated that GATA4 promoted cell invasion and migration, respectively. Western blotting and reverse transcription‑quantitative polymerase chain reaction revealed that GATA4 overexpression decreased the expression of epithelial markers and increased the expression of mesenchymal markers. By contrast, GATA4 inhibition increased the expression of epithelial markers and decreased the mesenchymal markers. Additionally, chromatin immunoprecipitation and dual‑luciferase reporter assays revealed that GATA4 promoted epithelial‑mesenchymal transition through transcriptionally activating SLUG. Cell counting kit‑8 and colony formation assays were performed to analyze the effect of GATA4 on cell proliferation. The results indicated that GATA4 facilitated cell proliferation in NPC. In conclusion, GATA4 acts as an oncogene and serves crucial roles in NPC and GATA4 may find a potential application as therapeutic option in NPC.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhou Y, Chang H and Yang B: GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG. Exp Ther Med 16: 5318-5326, 2018
APA
Zhou, Y., Chang, H., & Yang, B. (2018). GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG. Experimental and Therapeutic Medicine, 16, 5318-5326. https://doi.org/10.3892/etm.2018.6826
MLA
Zhou, Y., Chang, H., Yang, B."GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG". Experimental and Therapeutic Medicine 16.6 (2018): 5318-5326.
Chicago
Zhou, Y., Chang, H., Yang, B."GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5318-5326. https://doi.org/10.3892/etm.2018.6826