Open Access

MicroRNA‑146b induces the PI3K/Akt/NF‑κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN

  • Authors:
    • Li Zhao
    • Xue Rong Yang
    • Xu Han
  • View Affiliations

  • Published online on: December 12, 2018     https://doi.org/10.3892/etm.2018.7087
  • Pages: 1171-1181
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the function of microRNA‑146b on myocardial infarction and the mechanism. An MTT assay, Annexin V/propidium iodide (PI) apoptosis assay, ELISA kits, western blot analysis and a caspase‑3/8 activity assay were used to measure cell growth, vascular apoptosis inflammatory factors, and the B‑cell lymphoma 2‑associated X protein (Bax), phosphatase and tensin homolog (PTEN), phosphoinositide 3‑kinase (PI3K)/Akt/nuclear factor (NF)‑κB signaling pathway. The expression of microRNA‑146b was downregulated in the myocardial infarction rat model, compared with the control group. In an in vitro model of myocardial infarction, the downregulation of microRNA‑146b increased inflammatory factors, vascular apoptosis, caspase‑3/8 activity and the protein expression of Bax. MicroRNA‑146b reduced vascular apoptosis, caspase‑3/8 activity and the protein expression of Bax. MicroRNA‑146b also regulated the PI3K/Akt/NF‑κB signaling pathway to mediate vascular inflammation and apoptosis in myocardial infarction by PTEN. A PI3K inhibitor decreased the effect of microRNA‑146b on vascular inflammation and apoptosis following myocardial infarction. In conclusion, microRNA‑146b mediated vascular inflammation and apoptosis in patients with myocardial infarction, which may be associated with activation of the PI3K/Akt/NF‑κB signaling pathway by PTEN.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao L, Yang XR and Han X: MicroRNA‑146b induces the PI3K/Akt/NF‑κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN. Exp Ther Med 17: 1171-1181, 2019
APA
Zhao, L., Yang, X.R., & Han, X. (2019). MicroRNA‑146b induces the PI3K/Akt/NF‑κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN. Experimental and Therapeutic Medicine, 17, 1171-1181. https://doi.org/10.3892/etm.2018.7087
MLA
Zhao, L., Yang, X. R., Han, X."MicroRNA‑146b induces the PI3K/Akt/NF‑κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN". Experimental and Therapeutic Medicine 17.2 (2019): 1171-1181.
Chicago
Zhao, L., Yang, X. R., Han, X."MicroRNA‑146b induces the PI3K/Akt/NF‑κB signaling pathway to reduce vascular inflammation and apoptosis in myocardial infarction by targeting PTEN". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1171-1181. https://doi.org/10.3892/etm.2018.7087