Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways

  • Authors:
    • Huiyu Yue
    • Bin Hu
    • Zhikai Luo
    • Mei Liu
  • View Affiliations

  • Published online on: December 13, 2018     https://doi.org/10.3892/etm.2018.7098
  • Pages: 1463-1469
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Abstract

The aim of the current study was to investigate whether metformin could counteract sevoflurane‑induced neurotoxicity. In vitro experiments on the sevoflurane‑induced nerve injury were performed using hippocampal neurons. Neuronal apoptosis was detected by an MTT assay. Protein expression levels of apoptosis‑associated genes, including cleaved‑caspase‑3, apoptosis regulator BAX and apoptosis regulator Bcl‑2 were detected by western blot analysis. The mechanism of the effect of metformin on sevoflurane‑induced neuronal apoptosis was investigated using a sphingosine 1‑phosphate receptor 1 (S1P1) antagonist (VPC23019) and mitogen‑activated protein kinase kinase inhibitor (U0126). The current study revealed that metformin may reduce sevoflurane‑induced neuronal apoptosis via activating mitogen‑activated protein kinase (ERK)1/2 phosphorylation. VPC23019 and U0126 eliminated the neuroprotective effects of metformin on neuronal apoptosis, which suggests that metformin is able to protect against sevoflurane‑induced neurotoxicity via activation of the S1P1‑dependent ERK1/2 signaling pathway.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yue H, Hu B, Luo Z and Liu M: Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Exp Ther Med 17: 1463-1469, 2019
APA
Yue, H., Hu, B., Luo, Z., & Liu, M. (2019). Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Experimental and Therapeutic Medicine, 17, 1463-1469. https://doi.org/10.3892/etm.2018.7098
MLA
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17.2 (2019): 1463-1469.
Chicago
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1463-1469. https://doi.org/10.3892/etm.2018.7098