Open Access

Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration

  • Authors:
    • Mihai Bucur
    • Carolina Constantin
    • Monica Neagu
    • Sabina Zurac
    • Octavian Dinca
    • Cristian Vladan
    • Mirela Cioplea
    • Cristiana Popp
    • Luciana Nichita
    • Ecaterina Ionescu
  • View Affiliations

  • Published online on: December 6, 2018     https://doi.org/10.3892/etm.2018.7063
  • Pages: 982-989
  • Copyright: © Bucur et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Wound healing process comprises a complex network of cells and molecules that are regulated in order to pursue tissue regeneration. Our study focused on the capacity of alveolar blood clots (ABCs), platelet-rich fibrin (PRF) and plasma rich in growth factors (PRGF) to induce in vitro fibroblasts proliferation and migration as a measure of alveolar regeneration. Using cellular impedance with xCELLigence technology we quantified the proliferation and the migration capacity of L929 fibroblast standard cell line in the presence of 4 different ABCs and 3 different PRFs harvested from healthy individuals during standard tooth extraction. We obtained a clear cellular proliferation induced by the compounds mainly after 24 h of cultivation, in a dose-dependent manner. After 48 h of cultivation we registered activated proliferation, but slightly decreased compared to the 24 h profile. Our data confirm that the presence of the blood clot is involved in the regenerative processes. The migratory capacity of fibroblasts was statistically activated by the PL compounds while not affected by the tested PRFs. The chemical mediators present within the blood clot, either produced by inflammatory cells captive within, or by endothelial or mesenchymal cells induced fibroblastic proliferation and subsequent collagen deposition.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Bucur M, Constantin C, Neagu M, Zurac S, Dinca O, Vladan C, Cioplea M, Popp C, Nichita L, Ionescu E, Ionescu E, et al: Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Exp Ther Med 17: 982-989, 2019
APA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C. ... Ionescu, E. (2019). Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Experimental and Therapeutic Medicine, 17, 982-989. https://doi.org/10.3892/etm.2018.7063
MLA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17.2 (2019): 982-989.
Chicago
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17, no. 2 (2019): 982-989. https://doi.org/10.3892/etm.2018.7063