Open Access

Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy

  • Authors:
    • Changtai Wang
    • Shu Yu
    • Yafei Zhang
    • Min Zhang
    • Liying Lv
    • Cheng Huang
    • Xu Li
    • Jun Li
    • Zhenhua Zhang
  • View Affiliations

  • Published online on: February 11, 2019     https://doi.org/10.3892/etm.2019.7255
  • Pages: 2473-2484
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The association between hepatitis B virus (HBV) quasispecies (QS) and the efficacy of nucleos(t)ide analog therapy is currently not well defined, particularly in the case of lamivudine (LAM)/adefovir (ADV) combination rescue therapy for patients with chronic HBV infection (CHB) presenting with LAM resistance. In the present study, 16 CHB patients with the rtM204I/V mutation in the tyrosine-methionine-aspartate‑aspartate motif of the C domain of the polymerase gene who switched to LAM/ADV treatment due to LAM resistance were assessed. HBV DNA was isolated from these patients and the reverse transcriptase (RT) region was sequenced. The QS heterogeneity and distribution was analyzed, the mutation sites were recorded and the phylogenetic trees were constructed. The results indicated that QS heterogeneity and distribution in the RT and S regions were not significantly different between responders (RS) and non‑RS (NRS) at baseline (P>0.05), except for the higher frequency of a dominant strain in the RT region at the nucleotide level in the RS group (P=0.039). In addition, in NRS, no significant difference in QS heterogeneity or distribution in these regions was identified at six months vs. the baseline. Furthermore, although in the non‑responder group the frequency of the LAM resistance‑associated mutations (rtM204V/I) decreased at 6 months compared with the baseline, it did not disappear in any of the patients after six months of treatment. Analysis of individual patients did not indicate any consistent selection of specific HBV mutants during LAM/ADV rescue therapy. In conclusion, the baseline HBV QS within the RT and S regions may not be a valid predictor of the response to LAM/ADV rescue treatment in CHB patients with LAM resistance.
View Figures
View References

Related Articles

Journal Cover

April-2019
Volume 17 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Yu S, Zhang Y, Zhang M, Lv L, Huang C, Li X, Li J and Zhang Z: Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Exp Ther Med 17: 2473-2484, 2019
APA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C. ... Zhang, Z. (2019). Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Experimental and Therapeutic Medicine, 17, 2473-2484. https://doi.org/10.3892/etm.2019.7255
MLA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17.4 (2019): 2473-2484.
Chicago
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2473-2484. https://doi.org/10.3892/etm.2019.7255