Open Access

Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-κB pathway and inflammatory factors in the intestinal tract of rats with septic shock

  • Authors:
    • Wenhao Zhang
    • Jiakui Sun
    • Xiao Shen
    • Yinying Xue
    • Shoutao Yuan
    • Xiang Wang
  • View Affiliations

  • Published online on: February 6, 2019     https://doi.org/10.3892/etm.2019.7247
  • Pages: 2567-2574
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of Pseudomona blank s aeruginosa-mannose sensitive hemagglutini (PA-MSHA) preprocessing on toll like receptor (TLR)-4‑NF-κB pathway and inflammatory factors expression in the intestinal tract of rats with septic shock were investigated. A total of 30 rats were randomly divided into 3 groups (n=10): Blank control, septic shock, and PA-septic shock group. After the model was successfully established, the average arterial pressure in rats was monitored. The concentration of cytokine interleukin-l (IL-1), IL-6 and tumor necrosis factor-α (TNF-α) were determined by ELISA method. Flow cytometry was performed to detect TLR-4 expression. Number of in vitro chemotaxised neutrophils was detected by Transwell chamber. The expression of TLR-4, NF-κB and ICAM-1 and VCAM-1 was detected by western blot analysis. The concentration of cytokine IL-1, IL-6, TNF-α in the peritoneal lavage fluid and the intestinal tissue significantly increased in the septic shock group and the PA-septic shock group (P<0.05), and the concentration in the PA-septic shock group was significantly lower than that of the septic shock group (P<0.05). Compared to the control group, the expression of TLR-4, NF-κB and ICAM-1 and VCAM-1 increased in the septic shock and PA-septic shock group (P<0.05), and the expression level of PA-septic shock group was lower than the septic shock group (P<0.05). The expression of TLR-4 in the PA-septic shock group was lower than the septic shock group (P<0.05). PA-MSHA pretreatment reduced inflammation, thus preventing the intestinal injury caused by septic shock.
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April-2019
Volume 17 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang W, Sun J, Shen X, Xue Y, Yuan S and Wang X: Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-κB pathway and inflammatory factors in the intestinal tract of rats with septic shock. Exp Ther Med 17: 2567-2574, 2019
APA
Zhang, W., Sun, J., Shen, X., Xue, Y., Yuan, S., & Wang, X. (2019). Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-κB pathway and inflammatory factors in the intestinal tract of rats with septic shock. Experimental and Therapeutic Medicine, 17, 2567-2574. https://doi.org/10.3892/etm.2019.7247
MLA
Zhang, W., Sun, J., Shen, X., Xue, Y., Yuan, S., Wang, X."Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-κB pathway and inflammatory factors in the intestinal tract of rats with septic shock". Experimental and Therapeutic Medicine 17.4 (2019): 2567-2574.
Chicago
Zhang, W., Sun, J., Shen, X., Xue, Y., Yuan, S., Wang, X."Effect of PA-MSAH preprocessing on the expression of TLR-4-NF-κB pathway and inflammatory factors in the intestinal tract of rats with septic shock". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2567-2574. https://doi.org/10.3892/etm.2019.7247