Open Access

Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model

  • Authors:
    • Luanjun Tan
    • Yingdi Zhang
    • Yuanliang Huang
    • Yuan Luo
    • Yuehua Liu
  • View Affiliations

  • Published online on: February 22, 2019     https://doi.org/10.3892/etm.2019.7301
  • Pages: 2913-2920
  • Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hypoxia‑inducible factor (HIF)‑1α protein, which is upregulated by hypoxia, serves an important role in angiogenesis during osteogenesis. The aim of the present study was to investigate the effect of HIF‑1α on alveolar ridge preservation in a dog tooth extraction model. Six beagle dogs were used in the present study. The second and fourth premolar teeth of the lower jaws on both sides were extracted. Two unilateral extraction sockets were randomly selected and filled with Bio‑Oss and Bio‑Oss + HIF‑1α. The contralateral sockets remained unfilled and served as the negative control. Micro‑computed tomography examination and histological staining were performed to examine the difference of new bone formation among the three groups. Western blotting and reverse transcription‑quantitative polymerase chain reaction analysis were used to detect the expression levels of osteogenesis‑ and angiogenesis‑associated genes in the bone tissues of the three groups. Twelve weeks post‑surgery, trabecular bone formation in the Bio‑Oss + HIF‑1α group was significantly increased compared with the other groups. The expression levels of osteogenesis‑associated genes (runt‑related transcription factor 2, osteoblast‑specific transcription factor osterix and osteocalcin) and angiogenesis‑associated genes (HIF‑1α and vascular endothelial growth factor) were all significantly increased in the Bio‑Oss + HIF‑1α group compared with the other two groups (P<0.05). The present results indicated that Bio‑Oss with HIF‑1α can promote osteogenesis and angiogenesis in vivo and may be used as an effective treatment for the preservation of the alveolar ridge.
View Figures
View References

Related Articles

Journal Cover

April-2019
Volume 17 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tan L, Zhang Y, Huang Y, Luo Y and Liu Y: Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model. Exp Ther Med 17: 2913-2920, 2019
APA
Tan, L., Zhang, Y., Huang, Y., Luo, Y., & Liu, Y. (2019). Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model. Experimental and Therapeutic Medicine, 17, 2913-2920. https://doi.org/10.3892/etm.2019.7301
MLA
Tan, L., Zhang, Y., Huang, Y., Luo, Y., Liu, Y."Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model". Experimental and Therapeutic Medicine 17.4 (2019): 2913-2920.
Chicago
Tan, L., Zhang, Y., Huang, Y., Luo, Y., Liu, Y."Preservation of alveolar ridge after tooth extraction with hypoxia‑inducible factor‑1α protein in a dog model". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2913-2920. https://doi.org/10.3892/etm.2019.7301