Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia

  • Authors:
    • Jun‑Rong Lei
    • Xian‑Kun Tu
    • Yang Wang
    • De‑Wen Tu
    • Song‑Sheng Shi
  • View Affiliations

  • Published online on: February 27, 2019     https://doi.org/10.3892/etm.2019.7324
  • Pages: 3215-3221
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Abstract

Previous studies have demonstrated that inflammation and disruption of the blood‑brain barrier (BBB) are important pathological processes during focal cerebral ischemia. Therefore, the present study evaluated the neuroprotective effects of resveratrol against brain damage, inflammation and BBB disruption in rats with focal cerebral ischemia and assessed the potential underlying molecular mechanisms. Sprague‑Dawley rats underwent cerebral ischemia/reperfusion (IR) and then received intraperitoneal resveratrol (10 and 100 mg/kg) 2 h following the onset of ischemia. Following 24 h of ischemia, neurological deficit scores, cerebral infarctions, morphological characteristics, cerebral water content, myeloperoxidase (MPO) activity and Evans blue extravasation were assessed. Additionally, the protein expression levels of Toll‑like receptor 4 (TLR4) and nuclear factor (NF)‑κB p65 were detected using western blot analyses, the mRNA expression levels of cyclooxygenase‑2 (COX‑2) and matrix metalloproteinase‑9 (MMP‑9) were examined by reverse‑transcription polymerase chain reaction, and tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β blood levels were determined by ELISA. Resveratrol significantly reduced neurological deficit scores, cerebral infarct sizes, neuronal injury, MPO activity and EB content. Cerebral ischemia increased the expression levels of TLR4, NF‑κB p65, COX‑2, MMP‑9, TNF‑α and IL‑1β, but all of these factors were reduced by resveratrol. In conclusion, the present data suggest that resveratrol reduces inflammation, BBB disruption and brain damage in rats following focal cerebral ischemia. Additionally, the neuroprotective effects of resveratrol against cerebral ischemia may be associated with downregulation of the TLR4 pathway.
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April-2019
Volume 17 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lei JR, Tu XK, Wang Y, Tu DW and Shi SS: Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia. Exp Ther Med 17: 3215-3221, 2019
APA
Lei, J., Tu, X., Wang, Y., Tu, D., & Shi, S. (2019). Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia. Experimental and Therapeutic Medicine, 17, 3215-3221. https://doi.org/10.3892/etm.2019.7324
MLA
Lei, J., Tu, X., Wang, Y., Tu, D., Shi, S."Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia". Experimental and Therapeutic Medicine 17.4 (2019): 3215-3221.
Chicago
Lei, J., Tu, X., Wang, Y., Tu, D., Shi, S."Resveratrol downregulates the TLR4 signaling pathway to reduce brain damage in a rat model of focal cerebral ischemia". Experimental and Therapeutic Medicine 17, no. 4 (2019): 3215-3221. https://doi.org/10.3892/etm.2019.7324