Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease

  • Authors:
    • Shize Li
    • Nini Wang
    • Jiyu Lou
    • Xiaoman Zhang
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  • Published online on: March 27, 2015     https://doi.org/10.3892/etm.2015.2391
  • Pages: 2389-2393
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Abstract

The aim of this study was to investigate the expression of β-site APP-cleaving enzyme 1 (BACE1) in the hippocampal tissue of an insulin‑resistant rat model, and thereby explore the roles of BACE1 and insulin resistance (IR) in the pathogenesis of Alzheimer's disease (AD). A total of 36 male Sprague‑Dawley rats, aged 2 months, were randomly divided into three groups. These were an insulin‑resistant (experimental) group, a high fat control group and a blank control group. The cognitive function and behavioral changes of the rats were tested by a Morris water maze experiment. Amyloid β (Aβ) deposition was detected by an immunohistochemical method. The expression levels of BACE1 in the rat hippocampal tissues were detected by enzyme‑linked immunosorbent assay, western blotting and reverse transcription‑quantitative polymerase chain reaction technology. The rats in the experimental group had evident learning and memory impairment, with significantly decreased learning memory. The modeling was successful; in the experimental group, the rats exhibited IR and their glucose metabolism was significantly abnormal. However, there was no characteristic pathology of AD. The expression of BACE1 in the brain tissue of rats in the experimental group was significantly higher than that in high fat control and blank control groups (P<0.01). In conclusion, the expression of BACE1 in the brain tissue of insulin‑resistant rats increased, and IR was indicated to participate in the pathogenesis of AD.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li S, Wang N, Lou J and Zhang X: Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease. Exp Ther Med 9: 2389-2393, 2015
APA
Li, S., Wang, N., Lou, J., & Zhang, X. (2015). Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease. Experimental and Therapeutic Medicine, 9, 2389-2393. https://doi.org/10.3892/etm.2015.2391
MLA
Li, S., Wang, N., Lou, J., Zhang, X."Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease". Experimental and Therapeutic Medicine 9.6 (2015): 2389-2393.
Chicago
Li, S., Wang, N., Lou, J., Zhang, X."Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2389-2393. https://doi.org/10.3892/etm.2015.2391