Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.

  • Authors:
    • A Venditti
    • F Buccisano
    • G Del Poeta
    • A Battaglia
    • A Tamburini
    • A Bruno
    • G Adorno
    • T Caravita
    • S Santinelli
    • B Del Moro
    • A M Epiceno
    • G Ballatore
    • S Amadori
  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/ijmm.1.1.67
  • Pages: 67-137
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Abstract

Determination of CD34+ cells was performed in bone marrow and G-CSF mobilised peripheral blood samples. We adopted three different protocols of analysis: the Milan/Mulhouse protocol, the ISHAGE guidelines for CD34+ cell determination and our own protocol based upon the use of PAINT-A-GATEPRO software analysis program. An excellent correlation was demonstrated between the three methods (r2 0.98); however the analysis of variance showed a statistically significant difference between the results generated with the three methods (P=0.001). The differences between the three procedures are discussed with a special focus on the value of CD34+dim cells and the role of CD45 in the setting of a double staining. We have in fact identified a minor subset (CD34+CD38+CD45-) which would go unrecognised based upon its CD45 negativity.

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Jan 1998
Volume 1 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Venditti A, Buccisano F, Del Poeta G, Battaglia A, Tamburini A, Bruno A, Adorno G, Caravita T, Santinelli S, Del Moro B, Del Moro B, et al: Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.. Int J Mol Med 1: 67-137, 1998
APA
Venditti, A., Buccisano, F., Del Poeta, G., Battaglia, A., Tamburini, A., Bruno, A. ... Amadori, S. (1998). Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.. International Journal of Molecular Medicine, 1, 67-137. https://doi.org/10.3892/ijmm.1.1.67
MLA
Venditti, A., Buccisano, F., Del Poeta, G., Battaglia, A., Tamburini, A., Bruno, A., Adorno, G., Caravita, T., Santinelli, S., Del Moro, B., Epiceno, A., Ballatore, G., Amadori, S."Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.". International Journal of Molecular Medicine 1.1 (1998): 67-137.
Chicago
Venditti, A., Buccisano, F., Del Poeta, G., Battaglia, A., Tamburini, A., Bruno, A., Adorno, G., Caravita, T., Santinelli, S., Del Moro, B., Epiceno, A., Ballatore, G., Amadori, S."Multiparametric analysis for the enumeration of CD34+ cells from bone marrow and stimulated peripheral blood.". International Journal of Molecular Medicine 1, no. 1 (1998): 67-137. https://doi.org/10.3892/ijmm.1.1.67