Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone).

  • Authors:
    • H Kaneko
    • H Hibasami
    • N Satoh
    • H Wakabayashi
    • H Ikeda
    • N Tsuge
    • K Yonemaru
    • A Muraki
    • Y Kawarada
    • K Nakashima
  • View Affiliations

  • Published online on: June 1, 1998     https://doi.org/10.3892/ijmm.1.6.931
  • Pages: 931-937
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Abstract

Polyamines are considered to be important intracellular molecules for the proliferation of the cancer cells. In this study, effects of methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), a potent inhibitor of the polyamine biosynthetic pathway, on the growth and cell cycle of T-47D human breast cancer cells were investigated. MGBCP dose-dependently inhibited the growth of T-47D cells, in which the contents of spermine, spermidine and putrescine decreased concomitantly. The gene expression of cyclin D1 was also repressed by the MGBCP treatment. The MGBCP-treated cells clearly exhibited morphological changes indicating the blebbing and chromatin condensation which are characteristic of apoptosis. Flow cytometric analysis showed hypo-diploid subpopulations due to apoptotic cells, and characteristic oligonucleosomal-sized DNA fragments were clearly observed for MGBCP-treated cells as the concentration of the drug was increased. These findings suggest that the inhibition of polyamine synthesis results in the repressions of cyclin D1 expression and cell cycle progression, eventually inducing apoptosis in these human breast cancer cells.

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Jun 1998
Volume 1 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kaneko H, Hibasami H, Satoh N, Wakabayashi H, Ikeda H, Tsuge N, Yonemaru K, Muraki A, Kawarada Y, Nakashima K, Nakashima K, et al: Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone).. Int J Mol Med 1: 931-937, 1998
APA
Kaneko, H., Hibasami, H., Satoh, N., Wakabayashi, H., Ikeda, H., Tsuge, N. ... Nakashima, K. (1998). Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone).. International Journal of Molecular Medicine, 1, 931-937. https://doi.org/10.3892/ijmm.1.6.931
MLA
Kaneko, H., Hibasami, H., Satoh, N., Wakabayashi, H., Ikeda, H., Tsuge, N., Yonemaru, K., Muraki, A., Kawarada, Y., Nakashima, K."Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone).". International Journal of Molecular Medicine 1.6 (1998): 931-937.
Chicago
Kaneko, H., Hibasami, H., Satoh, N., Wakabayashi, H., Ikeda, H., Tsuge, N., Yonemaru, K., Muraki, A., Kawarada, Y., Nakashima, K."Involvement of apoptosis and cyclin D1 gene repression in growth inhibition of T-47D human breast cancer cells by methylglyoxal bis(cyclopentylamidinohydrazone).". International Journal of Molecular Medicine 1, no. 6 (1998): 931-937. https://doi.org/10.3892/ijmm.1.6.931