Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells

  • Authors:
    • Y. Ogawa
    • A. Nishioka
    • T. Kobayashi
    • S. Kariya
    • S. Hamasato
    • T. Saibara
    • K. Nakayama
    • H. Seguchi
    • S. Yoshida
  • View Affiliations

  • Published online on: September 1, 2002     https://doi.org/10.3892/ijmm.10.3.263
  • Pages: 263-268
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Abstract

We examined sequential changes in post-irradiated peripheral blood T cells taken from normal volunteers, using a microscopy-video system, mitochondrial membrane potential assay, annexin V, propidium iodide, and cytochrome c ELISA kit. After 5 Gy irradiation with 10 MV X-ray from a linear accelerator, the percentages of apoptotic T cells were estimated as approximately 5, 10, 20, 35, and 70%, at 0, 3, 6, 10, and 20 h after irradiation, respectively, as observed with the microscopy-video system. Using a CCD camera-equipped fluorescence microscope and MitoCapture, a mitochondrial membrane potential indicator, approximately half of the T cells showed dysfunction of mitochondrial membrane potential at 10 h after 5 Gy irradiation. With regard to annexin V and propidium iodide, approximately 40 and 5% of the human peripheral T cells showed positivity against annexin V and propidium iodide at that time, respectively. Mitochondrial cytochrome c release from the mitochondria to the cytosol was confirmed to start at 10 h and to reach a maximum at 20 h after 5 Gy of irradiation. These results demonstrated that mitochondrial cytochrome c release occurred following dysfunction of mitochondrial membrane potential in radiation-induced T cell apoptosis.

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September 2002
Volume 10 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ogawa Y, Nishioka A, Kobayashi T, Kariya S, Hamasato S, Saibara T, Nakayama K, Seguchi H and Yoshida S: Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells. Int J Mol Med 10: 263-268, 2002
APA
Ogawa, Y., Nishioka, A., Kobayashi, T., Kariya, S., Hamasato, S., Saibara, T. ... Yoshida, S. (2002). Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells. International Journal of Molecular Medicine, 10, 263-268. https://doi.org/10.3892/ijmm.10.3.263
MLA
Ogawa, Y., Nishioka, A., Kobayashi, T., Kariya, S., Hamasato, S., Saibara, T., Nakayama, K., Seguchi, H., Yoshida, S."Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells". International Journal of Molecular Medicine 10.3 (2002): 263-268.
Chicago
Ogawa, Y., Nishioka, A., Kobayashi, T., Kariya, S., Hamasato, S., Saibara, T., Nakayama, K., Seguchi, H., Yoshida, S."Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells". International Journal of Molecular Medicine 10, no. 3 (2002): 263-268. https://doi.org/10.3892/ijmm.10.3.263