Molecular analysis of TGF-βs and their receptors in human keratinocyte cell lines of different biological behaviour

  • Authors:
    • Beatrice E. Bachmeier
    • Isabell Ruoss
    • Hjalmar G. Hagedorn
    • Andreas G. Nerlich
  • View Affiliations

  • Published online on: October 1, 2002     https://doi.org/10.3892/ijmm.10.4.371
  • Pages: 371-376
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We investigated three clonally related human keratinocyte cell lines of different biological behaviour, HaCaT (non-tumorigenic), A5 (benign, tumorigenic) and II-4RT (malignant, tumorigenic), with regard to the expression of TGF-β-isoforms -1, -2 and -3 and that of the TGF-β-cell-receptors TBR-I, -II and -III. In addition, we amplified and sequenced the genome of TBR-II which is known to be a target for mutations in several types of malignant tumors including squamous cell carcinomas. In all three cell lines, TGF-β1 and -β3 were present only in very low amounts. Western blots provided no evidence for differences in TGF-β1 between the cell lines. However, in immunohistochemistry more cells were slightly positive for this cytokine in HaCaT than in A5 and II-4RT cells. In contrast, a significantly variable expression of TGF-β2 was seen by both Western blot and immunohistochemistry. Thereby, the non-tumorigenic HaCaT-cells contained significantly more TGF-β2 than the tumorigenic, benign A5 cells and the malignant II-4RT cells. TBR-I, -II and -III were present in all three cell lines. While most cells were positive for TBR-I, only part of the cells contained TBR-II and -III, however, without obvious differences between the three cell lines. The molecular analysis of all 7 exons of TBR-II by PCR amplification and direct sequencing revealed in all three cell lines correct sequences without evidence for mutations. Our study indicates differences in the expression of TGF-β in a human model of keratinocytes of varying tumorigenicity, but presents no evidence for mutations in the functionally most important TGF-β-receptor TBR-II. This suggests a dysregulation of cytokine control on the level of TGF-β expression, which may be responsible for the biological behaviour.

Related Articles

Journal Cover

October 2002
Volume 10 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bachmeier BE, Ruoss I, Hagedorn HG and Nerlich AG: Molecular analysis of TGF-βs and their receptors in human keratinocyte cell lines of different biological behaviour. Int J Mol Med 10: 371-376, 2002
APA
Bachmeier, B.E., Ruoss, I., Hagedorn, H.G., & Nerlich, A.G. (2002). Molecular analysis of TGF-βs and their receptors in human keratinocyte cell lines of different biological behaviour. International Journal of Molecular Medicine, 10, 371-376. https://doi.org/10.3892/ijmm.10.4.371
MLA
Bachmeier, B. E., Ruoss, I., Hagedorn, H. G., Nerlich, A. G."Molecular analysis of TGF-βs and their receptors in human keratinocyte cell lines of different biological behaviour". International Journal of Molecular Medicine 10.4 (2002): 371-376.
Chicago
Bachmeier, B. E., Ruoss, I., Hagedorn, H. G., Nerlich, A. G."Molecular analysis of TGF-βs and their receptors in human keratinocyte cell lines of different biological behaviour". International Journal of Molecular Medicine 10, no. 4 (2002): 371-376. https://doi.org/10.3892/ijmm.10.4.371