Effect of IL-1β, TNF-agr; and IL-4 on complement regulatory protein mRNA expression in human articular chondrocytes

  • Authors:
    • Anna Hyc
    • Anna Osiecka-Iwan
    • Piotr Strzelczyk
    • Stanislaw Moskalewski
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/ijmm.11.1.91
  • Pages: 91-94
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Abstract

Most of the cells possess complement regulatory proteins (CRPs) that protect them against complement-mediated damage. In our previous work we revealed that human articular chondrocytes express CPRs. Moreover, increase of CRPs expression after treatment of chondrocytes with proinflammatory cytokines IL-1β and TNF-α has been demonstrated by ELISA technique. Chondroprotective cytokine IL-4 stimulated expression of CD46 only. In this work RT-PCR technique was used to evaluate the expression of mRNA of cell surface CPRs in cultured isolated articular human chondrocytes after treatment with IL-1β, TNF-α and IL-4. Chondrocytes stimulated with IL-1β and TNF-α showed augmented levels of CD46, CD55 and CD59 mRNA. Treatment with IL-4, however, increased only the level of CD46 mRNA. These results confirm and extend our previous observations. CD35 mRNA was not found. Expression of complement regulatory proteins on chondrocytes and its upregulation by cytokines stimulating matrix degradation could be important for the protection of these cells against complement-mediated lysis, which might be caused by immunocomplexes deposited in articular cartilage in inflammatory joint diseases.

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January 2003
Volume 11 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hyc A, Osiecka-Iwan A, Strzelczyk P and Moskalewski S: Effect of IL-1β, TNF-agr; and IL-4 on complement regulatory protein mRNA expression in human articular chondrocytes. Int J Mol Med 11: 91-94, 2003
APA
Hyc, A., Osiecka-Iwan, A., Strzelczyk, P., & Moskalewski, S. (2003). Effect of IL-1β, TNF-agr; and IL-4 on complement regulatory protein mRNA expression in human articular chondrocytes. International Journal of Molecular Medicine, 11, 91-94. https://doi.org/10.3892/ijmm.11.1.91
MLA
Hyc, A., Osiecka-Iwan, A., Strzelczyk, P., Moskalewski, S."Effect of IL-1β, TNF-agr; and IL-4 on complement regulatory protein mRNA expression in human articular chondrocytes". International Journal of Molecular Medicine 11.1 (2003): 91-94.
Chicago
Hyc, A., Osiecka-Iwan, A., Strzelczyk, P., Moskalewski, S."Effect of IL-1β, TNF-agr; and IL-4 on complement regulatory protein mRNA expression in human articular chondrocytes". International Journal of Molecular Medicine 11, no. 1 (2003): 91-94. https://doi.org/10.3892/ijmm.11.1.91